PMID- 12656904
OWN - NLM
STAT- MEDLINE
DCOM- 20030602
LR  - 20190906
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 91
IP  - 6
DP  - 2003 Apr
TI  - Inverse expression of uroplakins and inducible nitric oxide synthase in the 
      urothelium of patients with bladder outlet obstruction.
PG  - 507-12
AB  - OBJECTIVE: To assess the expression and distribution of uroplakins, protein 
      subunits of the asymmetric unit membrane (AUM), and inducible nitric-oxide 
      synthase (iNOS) in the urinary bladder urothelium of patients with bladder outlet 
      obstruction (BOO) caused by benign prostatic hyperplasia (BPH). PATIENTS AND 
      METHODS: Urinary bladder urothelium samples from 15 men (mean age 69 years) with 
      BOO secondary to BPH were processed for light and electron immunocytochemistry. 
      Uroplakins and iNOS were detected, and areas of apical surface covered with AUM 
      were compared with those of iNOS-positive urothelial cells. RESULTS: Areas of 
      superficial urothelial cells with no AUM were found in all obstructed bladder 
      samples. The immuno-electron microscopy showed that the uroplakin-positive cells 
      had the characteristic appearance of terminally differentiated umbrella cells, 
      whereas cells from the uroplakin-negative regions were undifferentiated, 
      typically showing microvilli on their apical surface. iNOS was not detected in 
      areas with continuous AUM staining, but was readily detected in the 
      uroplakin-negative areas. There was an inverse correlation between the intensity 
      of uroplakin and iNOS staining. CONCLUSIONS: In patients with BOO associated with 
      BPH, some superficial urothelial cells lacked the AUM, suggesting focal 
      compromise of the blood-urine permeability barrier. In such relatively 
      undifferentiated urothelial zones there was an accompanying increase in the 
      expression of iNOS, which marks perturbed urothelial differentiation and may 
      modulate bladder response to the outlet obstruction.
FAU - Romih, R
AU  - Romih R
AD  - Institute of Cell Biology, Medical Faculty, University of Ljubljana, Ljubljana, 
      Slovenia. rok.romih@mf.uni-lj.si
FAU - Korosec, P
AU  - Korosec P
FAU - Jezernik, K
AU  - Jezernik K
FAU - Sedmak, B
AU  - Sedmak B
FAU - Trsinar, B
AU  - Trsinar B
FAU - Deng, F-M
AU  - Deng FM
FAU - Liang, F-X
AU  - Liang FX
FAU - Sun, T-T
AU  - Sun TT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (UPK1A protein, human)
RN  - 0 (UPK2 protein, human)
RN  - 0 (Uroplakin II)
RN  - 0 (Uroplakin Ia)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Membrane Glycoproteins/*metabolism
MH  - Membrane Proteins/*metabolism
MH  - Middle Aged
MH  - Nitric Oxide Synthase/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Prostatic Hyperplasia/metabolism/surgery
MH  - Transurethral Resection of Prostate/methods
MH  - Urinary Bladder/ultrastructure
MH  - Urinary Bladder Neck Obstruction/*metabolism/pathology
MH  - Uroplakin II
MH  - Uroplakin Ia
MH  - Urothelium/metabolism/pathology
EDAT- 2003/03/27 05:00
MHDA- 2003/06/05 05:00
CRDT- 2003/03/27 05:00
PHST- 2003/03/27 05:00 [pubmed]
PHST- 2003/06/05 05:00 [medline]
PHST- 2003/03/27 05:00 [entrez]
AID - 3052 [pii]
AID - 10.1046/j.1464-410x.2003.03052.x [doi]
PST - ppublish
SO  - BJU Int. 2003 Apr;91(6):507-12. doi: 10.1046/j.1464-410x.2003.03052.x.