PMID- 12658681
OWN - NLM
STAT- MEDLINE
DCOM- 20031119
LR  - 20081121
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 24
IP  - 6
DP  - 2003 Mar
TI  - Open-tubular capillary electrochromatography using a polymeric surfactant 
      coating.
PG  - 945-51
AB  - A stable polyelectrolyte multilayer (PEM) coating was investigated for use in 
      open-tubular capillary electrochromatography (o-CEC). In this approach, the PEM 
      consisted of the cationic polymer of a quaternary ammonium salt, 
      poly(diallyldimethylammonium chloride) and the anionic polymeric surfactant, 
      poly(sodium undecylenic sulfate). Both the cationic and anionic polymers were 
      physically adsorbed to the surface of a fused-silica capillary by use of a simple 
      coating procedure. This procedure involved an alternate rinse of the positively 
      and negatively charged polymers. The performance of the PEM coating as a dynamic 
      stationary phase was evaluated by use of electrochromatographic experiments and 
      showed good selectivity for both phenols and benzodiazepines. Reproducibility of 
      the PEM coating was also evaluated by calculating the relative standard 
      deviations (RSDs) of the electroosomotic flow (EOF). The run-to-run and 
      capillary-to-capillary RSD values of the EOF were less than 1.5%. The endurance 
      of the coating was more than 100 runs. The importance of the PEM coating was 
      illustrated by comparing separations on a bare uncoated capillary with the coated 
      capillary. In addition, the chromatographic performance using o-CEC and micellar 
      electrokinetic chromatography (MEKC) was compared for the separation of 
      benzodiazepines.
FAU - Kamande, Mary W
AU  - Kamande MW
AD  - Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
FAU - Kapnissi, Constantina P
AU  - Kapnissi CP
FAU - Zhu, Xiaofeng
AU  - Zhu X
FAU - Akbay, Cevdet
AU  - Akbay C
FAU - Warner, Isiah M
AU  - Warner IM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Buffers)
RN  - 0 (Phenols)
RN  - 0 (Polymers)
RN  - 0 (Surface-Active Agents)
RN  - 12794-10-4 (Benzodiazepines)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Benzodiazepines/*chemistry/isolation & purification
MH  - Buffers
MH  - Electrophoresis, Capillary/*methods
MH  - Phenols/chemistry/isolation & purification
MH  - Polymers/*chemistry
MH  - Pressure
MH  - Reproducibility of Results
MH  - Silicon Dioxide/chemistry
MH  - Surface-Active Agents/*chemistry
EDAT- 2003/03/27 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/03/27 05:00
PHST- 2003/03/27 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/03/27 05:00 [entrez]
AID - 10.1002/elps.200390137 [doi]
PST - ppublish
SO  - Electrophoresis. 2003 Mar;24(6):945-51. doi: 10.1002/elps.200390137.