PMID- 12659811
OWN - NLM
STAT- MEDLINE
DCOM- 20031210
LR  - 20190906
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 81
IP  - 3
DP  - 2003 Mar
TI  - The telomeric region of BTA18 containing a potential QTL region for health in 
      cattle exhibits high similarity to the HSA19q region in humans.
PG  - 270-8
AB  - We have applied a targeted physical mapping approach, based on the isolation of 
      bovine region-specific large-insert clones using homologous human sequences and 
      chromosome microdissection, to enhance the physical gene map of the telomeric 
      region of BTA18 and to prove its evolutionary conservation. The latter is a 
      prerequisite to exploit the dense human gene map for future positional cloning 
      approaches. Partial sequencing and homology search were used to characterize 20 
      BACs targeted to the BTA18q2.4-q2.6 region. We used fluorescence in situ 
      hybridization (FISH) to create physical maps of 11 BACs containing 15 gene loci; 
      these BACs served as anchor loci. Using these approaches, 12 new gene loci (CKM, 
      STK13, PSCD2, IRF3, VASP, ACTN4, ITPKC, CYP2B6, FOSB, DMPK, MIA, SIX5) were 
      assigned on BTA18 in the bovine cytogenetic map. A resolved physical map of 
      BTA18q2.4-q2.6 was developed, which encompasses 28 marker loci and a comparative 
      cytogenetic map that contains 15 genes. The mapping results demonstrate the high 
      evolutionary conservation between the telomeric region of BTA18q and HSA19q.
FAU - Brunner, Ronald M
AU  - Brunner RM
AD  - Research Unit for Molecular Biology, Research Institute for the Biology of Farm 
      Animals Dummerstorf, D-18196 Dummerstorf, Germany.
FAU - Sanftleben, Hanka
AU  - Sanftleben H
FAU - Goldammer, Tom
AU  - Goldammer T
FAU - Kuhn, Christa
AU  - Kuhn C
FAU - Weikard, Rosemarie
AU  - Weikard R
FAU - Kata, Srinivas R
AU  - Kata SR
FAU - Womack, James E
AU  - Womack JE
FAU - Schwerin, Manfred
AU  - Schwerin M
LA  - eng
SI  - GENBANK/AJ459269
SI  - GENBANK/AJ459270
SI  - GENBANK/AJ459271
SI  - GENBANK/AJ459272
SI  - GENBANK/AJ459273
SI  - GENBANK/AJ459274
SI  - GENBANK/AJ459275
SI  - GENBANK/AJ459276
SI  - GENBANK/AJ459277
SI  - GENBANK/AJ459278
SI  - GENBANK/AJ459279
SI  - GENBANK/AJ459280
SI  - GENBANK/AJ459281
SI  - GENBANK/AJ459282
SI  - GENBANK/AJ459283
SI  - GENBANK/AJ459284
SI  - GENBANK/AJ459285
SI  - GENBANK/AJ459286
SI  - GENBANK/AJ459287
SI  - GENBANK/AJ459288
SI  - GENBANK/AJ459289
SI  - GENBANK/AJ459290
SI  - GENBANK/AJ459291
SI  - GENBANK/AJ459292
SI  - GENBANK/AJ459293
SI  - GENBANK/AJ459294
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle/*genetics
MH  - DNA Primers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Physical Chromosome Mapping/*veterinary
MH  - *Quantitative Trait Loci
MH  - *Telomere
EDAT- 2003/03/28 05:00
MHDA- 2003/12/12 05:00
CRDT- 2003/03/28 05:00
PHST- 2003/03/28 05:00 [pubmed]
PHST- 2003/12/12 05:00 [medline]
PHST- 2003/03/28 05:00 [entrez]
AID - S0888754303000193 [pii]
AID - 10.1016/s0888-7543(03)00019-3 [doi]
PST - ppublish
SO  - Genomics. 2003 Mar;81(3):270-8. doi: 10.1016/s0888-7543(03)00019-3.