PMID- 12660029
OWN - NLM
STAT- MEDLINE
DCOM- 20030507
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 142
IP  - 1
DP  - 2003 Apr 1
TI  - Evaluation of chromosome 8 and 11 aneuploidies in washings and biopsy materials 
      of bladder transitional cell carcinoma.
PG  - 25-9
AB  - We compared chromosome 8 and 11 aneuploidies on bladder biopsy tumor tissues and 
      bladder washing samples of transitional cell carcinoma (TCC) and their 
      relationship to tumor malignancy. Interphase fluorescence in situ hybridization 
      (FISH) was applied to nuclei of washing material and biopsy samples of 17 
      patients with TCC. Incidence of cells having aneuploidy was clearly nonrandom 
      from patient to patient. There was no significant difference in the incidence of 
      aneuploid frequency for chromosomes 8 and 11 between biopsies of bladder tumors 
      and bladder washing samples (P > 0.05). For chromosome 8, incidence of disomic 
      cells (having two signals) in grade III tumors was significantly lower than in 
      grade II tumors of both washing samples (P = 0.004) and biopsy materials (P = 
      0.005), indicating a high frequency of aneuploidy. The incidence of nuclei with 
      four or more than four signals of chromosome 8 was significantly higher in grade 
      III tumors than in grade II tumors in washing samples (P = 0.031 and 0.003, 
      respectively). Similarly, in biopsy material, the incidence of nuclei with more 
      than four signals of chromosome 8 was significantly higher in grade III tumors 
      than in grade II tumors (P = 0.004). For chromosome 11, in both washing samples 
      and biopsy materials, the incidence of disomic cells (having two signals) in 
      grade III tumors was significantly lower than that detected in grade II tumors (P 
      = 0.031 and 0.014, respectively), indicating a high frequency of aneuploidy. In 
      biopsy materials, the incidence of nuclei with three or four signals was 
      significantly higher than that in grade II tumors (P = 0.014 and 0.012, 
      respectively). These findings suggest that FISH analysis of bladder washing 
      samples can be effectively detected as genetic changes of bladder tumors. It 
      might predict genetic progression of these tumors, which might be related to 
      tumor stage, because higher stages of tumors showed a higher incidence of 
      aneuploidies of chromosomes 8 and 11.
FAU - Acar, Hasan
AU  - Acar H
AD  - Department of Medical Genetics, Selcuk University, Medical Faculty, Konya 42080, 
      Turkey. yaseminsena@hotmail.com
FAU - Kilinc, Mehmet
AU  - Kilinc M
FAU - Yildirim, M Selman
AU  - Yildirim MS
FAU - Kaynak, Murat
AU  - Kaynak M
FAU - Cenker, Ali
AU  - Cenker A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Biopsy
MH  - Carcinoma, Transitional Cell/*genetics/pathology
MH  - Cell Nucleus/genetics
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Therapeutic Irrigation
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2003/03/28 05:00
MHDA- 2003/05/08 05:00
CRDT- 2003/03/28 05:00
PHST- 2003/03/28 05:00 [pubmed]
PHST- 2003/05/08 05:00 [medline]
PHST- 2003/03/28 05:00 [entrez]
AID - S0165460802008038 [pii]
AID - 10.1016/s0165-4608(02)00803-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Apr 1;142(1):25-9. doi: 
      10.1016/s0165-4608(02)00803-8.