PMID- 12660032
OWN - NLM
STAT- MEDLINE
DCOM- 20030507
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 142
IP  - 1
DP  - 2003 Apr 1
TI  - Detection by fluorescence in situ hybridization of microdeletions at 1p36 in 
      lymphomas, unidentified on cytogenetic analysis.
PG  - 46-50
AB  - The chromosomal band 1p36 exhibits frequent loss of heterozygosity in a variety 
      of human malignancies, suggesting the presence of an as yet unidentified tumor 
      suppressor gene. The faint terminal subbands often make cytogenetic analysis of 
      1p36 particularly difficult. Small deletions at this locus may therefore escape 
      detection on analysis by conventional cytogenetics, a hypothesis that we have 
      explored using fluorescence in situ hybridization (FISH) in malignant lymphoma. 
      The study cohort consisted of 20 cases of lymphoma of various subtypes without 
      any 1p abnormality on G-banded karyotyping. FISH was performed using a human 
      chromosome 1 paint and a bacterial artificial chromosome probe RP4-755G5 
      localizing to 1p36.33, the most telomeric subband of 1p36. Tumors demonstrating 
      1p36.33 deletions were additionally analyzed by FISH using a second probe from 
      the proximal 1p36.1 subband, to further define the breakpoint. Eight cases of 
      follicular lymphoma (FL), 5 diffuse large B-cell lymphomas (DLBCL), 2 Hodgkin 
      disease, 2 B-cell small lymphocytic lymphomas, 2 T-cell lymphomas, and 1 marginal 
      zone lymphoma were analyzed. FISH identified deletions at 1p36.33 in 5 of the 20 
      cases: 3 DLBCL and 2 FL. FISH is considerably more sensitive for identifying 
      lymphoma genetic alterations than conventional cytogenetics. Deletion of the 
      distal part of the 1p36 may be a much more common aberration than previously 
      recognized in lymphoma.
FAU - Rajgopal, Achuthan
AU  - Rajgopal A
AD  - Molecular Medicine Unit, St. James's University Hospital, Leeds LS9 7TF, UK.
FAU - Carr, Ian M
AU  - Carr IM
FAU - Leek, Jack P
AU  - Leek JP
FAU - Hodge, Donald
AU  - Hodge D
FAU - Bell, Sandra M
AU  - Bell SM
FAU - Roberts, Paul
AU  - Roberts P
FAU - Horgan, Kieran
AU  - Horgan K
FAU - Bonthron, David T
AU  - Bonthron DT
FAU - Selby, Peter J
AU  - Selby PJ
FAU - Markham, Alexander F
AU  - Markham AF
FAU - MacLennan, Kenneth A
AU  - MacLennan KA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 1
MH  - Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma/*genetics
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
EDAT- 2003/03/28 05:00
MHDA- 2003/05/08 05:00
CRDT- 2003/03/28 05:00
PHST- 2003/03/28 05:00 [pubmed]
PHST- 2003/05/08 05:00 [medline]
PHST- 2003/03/28 05:00 [entrez]
AID - S0165460802007409 [pii]
AID - 10.1016/s0165-4608(02)00740-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Apr 1;142(1):46-50. doi: 
      10.1016/s0165-4608(02)00740-9.