PMID- 12667605
OWN - NLM
STAT- MEDLINE
DCOM- 20031016
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1621
IP  - 1
DP  - 2003 Apr 7
TI  - Ni2+ enhances Fe2+/peroxide-induced oxidation of arachidonic acid and formation 
      of geno/cytotoxic 4-hydroxynonenal: a possible contributory mechanism in nickel 
      toxicity and allergenicity.
PG  - 9-16
AB  - Ni(2+), a toxic, carcinogenic and allergenic agent, affected both the kinetic and 
      chemical courses of the Fe(2+)-induced oxidation of arachidonic acid (AA) in 0.05 
      M phosphate buffer (pH 7.4) and at 37 degrees C. At 10 microM concentration, 
      Ni(2+) decreased the rate of oxidation of peroxide-free AA (200 microM) promoted 
      by 50 microM Fe(2+), as determined by measurement of thiobarbituric acid reactive 
      species (TBARS) and 1H NMR analysis. However, in the presence of low levels of 
      peroxides (e.g. 2%), Ni(2+) exerted a significant stimulatory effect on 
      Fe(2+)-induced AA oxidation and TBARS formation. 1H NMR analysis showed that 
      Ni(2+) (10 microM) enhanced formation of genotoxic alkenals including 
      4-hydroxy-2-nonenal (4-HNE, GC/MS evidence) by Fe(2+)-promoted degradation of 
      both AA and 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) methyl 
      esters. The observed stimulatory effects of Ni(2+) on peroxide breakdown and 
      cytotoxic aldehyde formation provide an attractive explanation to the enhanced 
      sensitization capacity of nickel in inflammatory states compared to normal 
      states.
FAU - Manini, Paola
AU  - Manini P
AD  - Department of Organic Chemistry and Biochemistry, University of Naples Federico 
      II, Via Cinthia 4, I-80126, Naples, Italy.
FAU - Napolitano, Alessandra
AU  - Napolitano A
FAU - Camera, Emanuela
AU  - Camera E
FAU - Caserta, Teresa
AU  - Caserta T
FAU - Picardo, Mauro
AU  - Picardo M
FAU - Palumbo, Anna
AU  - Palumbo A
FAU - d'Ischia, Marco
AU  - d'Ischia M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Aldehydes)
RN  - 0 (Cations, Divalent)
RN  - 0 (Leukotrienes)
RN  - 0 (Lipid Peroxides)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 67675-14-3 (15-hydroperoxy-5,8,11,13-eicosatetraenoic acid)
RN  - 7OV03QG267 (Nickel)
RN  - E1UOL152H7 (Iron)
RN  - K1CVM13F96 (4-hydroxy-2-nonenal)
SB  - IM
MH  - Aldehydes/*chemical synthesis
MH  - Arachidonic Acid/*chemistry
MH  - Cations, Divalent
MH  - Gas Chromatography-Mass Spectrometry
MH  - Iron/*chemistry/pharmacology
MH  - Leukotrienes/chemistry
MH  - Lipid Peroxidation
MH  - Lipid Peroxides/chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Models, Chemical
MH  - Nickel/*chemistry/pharmacology
MH  - Oxidation-Reduction/drug effects
MH  - Thiobarbituric Acid Reactive Substances/analysis
EDAT- 2003/04/02 05:00
MHDA- 2003/10/17 05:00
CRDT- 2003/04/02 05:00
PHST- 2003/04/02 05:00 [pubmed]
PHST- 2003/10/17 05:00 [medline]
PHST- 2003/04/02 05:00 [entrez]
AID - S0304416503000102 [pii]
AID - 10.1016/s0304-4165(03)00010-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2003 Apr 7;1621(1):9-16. doi: 
      10.1016/s0304-4165(03)00010-2.