PMID- 12670331
OWN - NLM
STAT- MEDLINE
DCOM- 20030630
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 121
IP  - 1
DP  - 2003 Apr
TI  - Detection by the fluorescence in situ hybridization technique of MYC 
      translocations in paraffin-embedded lymphoma biopsy samples.
PG  - 49-56
AB  - The detection of chromosomal translocations by fluorescence in situ hybridization 
      (FISH) is widely performed, but very few studies have attempted to apply this 
      technique to paraffin-embedded routine biopsy samples. We report the analysis of 
      paraffin sections from 36 B-cell lymphoma biopsies for MYC translocation 
      breakpoints by FISH. The probes consisted of multi-YAC constructs that flanked 
      the breakpoint region and that, therefore, separate upon a chromosomal 
      translocation and generate split (or "segregated") signals (rather than a more 
      ambiguous "co-localization" pattern, obtained when the two partners in a hybrid 
      gene are detected). The results were assessed by a simple approach that avoids 
      the counting of signal numbers per nucleus and so is appropriate for use in 
      routine practice. A total of 19 of the 36 lymphomas were scored as positive for 
      MYC translocation and this included 16 of the 20 patients in whom classic 
      cytogenetics had shown the presence of the (8;14) translocation (or one of its 
      two variants). We conclude that this two-colour "split-signal" technique based on 
      breakpoint flanking probes can readily detect chromosomal translocations in 
      paraffin sections. Furthermore, our results suggest that cases categorized as 
      "atypical Burkitt's/Burkitt-like" lymphoma (at least for adult patients) are 
      heterogeneous with respect to translocations involving the MYC oncogene, as well 
      as immunophenotype and clinical features.
FAU - Haralambieva, Eugenia
AU  - Haralambieva E
AD  - Leukaemia Research Fund Immunodiagnostics Unit, Nuffield Department of Clinical 
      Laboratory Sciences, John Radcliffe Hospital, Oxford OX3 9DU, UK.
FAU - Banham, Alison H
AU  - Banham AH
FAU - Bastard, Christian
AU  - Bastard C
FAU - Delsol, Georges
AU  - Delsol G
FAU - Gaulard, Philippe
AU  - Gaulard P
FAU - Ott, German
AU  - Ott G
FAU - Pileri, Stefano
AU  - Pileri S
FAU - Fletcher, Jonathan A
AU  - Fletcher JA
FAU - Mason, David Y
AU  - Mason DY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy
MH  - Burkitt Lymphoma/genetics
MH  - Female
MH  - *Genes, myc
MH  - Genetic Markers
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - *Translocation, Genetic
EDAT- 2003/04/03 05:00
MHDA- 2003/07/02 05:00
CRDT- 2003/04/03 05:00
PHST- 2003/04/03 05:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2003/04/03 05:00 [entrez]
AID - 4238 [pii]
AID - 10.1046/j.1365-2141.2003.04238.x [doi]
PST - ppublish
SO  - Br J Haematol. 2003 Apr;121(1):49-56. doi: 10.1046/j.1365-2141.2003.04238.x.