PMID- 12673589
OWN - NLM
STAT- MEDLINE
DCOM- 20030916
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 20
IP  - 2
DP  - 2003 Apr
TI  - [Detection of common chromosome abnormalities in myelodysplastic syndrome with a 
      panel fluorescence in situ hybridization].
PG  - 160-3
AB  - OBJECTIVE: To evaluate the value of a panel fluorescence in situ hybridization 
      (FISH) in the detection of common chromosome abnormalities in myelodysplastic 
      syndrome (MDS). METHODS: Twenty cases of MDS patients, whose karyotypes were 
      unknown by the FISH examiner beforehand, were analyzed with a panel FISH using 
      YAC248F5 (5q31), YAC938G5 (7q32), CEP8 and YAC 912C3 (20q12) probes to detect the 
      frequently occurring chromosome abnormalities (-5/5q, -/7q-, +8, 20q-) in MDS. 
      Then the results were compared to those of conventional cytogenetics (CC). 
      RESULTS: Among 20 cases, 13 cases were found to carry common chromosome 
      abnormalities by panel FISH (trisomy 8, five cases; -5/5q-, one case; 20q-, five 
      cases; 5q- accompanying 20q-, one case; complex abnormalities, one case). 
      However, on CC examination, only five cases were found to have common chromosomal 
      abnormalities (20q-, four cases; 5q- accompanying 20q-, one case). In addition, 
      trisomy 21, marker chromosome and complex abnormalities comprising -5, -7 and 
      marker chromosomes were seen in one case each, the rest were normal. CONCLUSION: 
      Panel FISH is a useful tool of molecular cytogenetics in the detection of common 
      chromosome abnormalities in MDS.
FAU - Shen, Yongmei
AU  - Shen Y
AD  - First Affiliated Hospital of Soochow University, Jiangsu Institue of Hematology, 
      Suzhou, Jiangsu, 215006 P. R. China. uujihsmc@public1.sz.js.cn
FAU - Xue, Yongquan
AU  - Xue Y
FAU - Li, Jianyong
AU  - Li J
FAU - Pan, Jinlan
AU  - Pan J
FAU - Wu, Yafang
AU  - Wu Y
FAU - Chen, Suning
AU  - Chen S
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 20/genetics
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
EDAT- 2003/04/04 05:00
MHDA- 2003/09/17 05:00
CRDT- 2003/04/04 05:00
PHST- 2003/04/04 05:00 [pubmed]
PHST- 2003/09/17 05:00 [medline]
PHST- 2003/04/04 05:00 [entrez]
AID - 940602045 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Apr;20(2):160-3.