PMID- 12674637
OWN - NLM
STAT- MEDLINE
DCOM- 20030710
LR  - 20111117
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 18
IP  - 6
DP  - 2002 Nov
TI  - [Expression of porcine interferon-gamma gene in Pichia pastoris and its effect of 
      inhibiting porcine reproductive and respiratory syndrome virus].
PG  - 683-6
AB  - In order to develop recombinant porcine Interferon-gamma (rPoIFN-gamma) to 
      prevent porcine viral infection, PoIFN-gamma cDNA lacking the signal peptide was 
      expressed in Pichia pastoris GS115 strain, and the effect of rPoIFN-gamma on 
      porcine reproductive and respiratory syndrome virus (PRRSV) was investigated. The 
      PoIFN-gamma gene was inserted into integrative vector pHIL-S1, and the 
      recombinant GS115 strain (pHIL-S1/PoIFN-gamma) was constructed by homologues 
      recombinant. The rPoIFN-gamma protein was 18 kD with an expressing yield of 18% 
      was assayed by SDS-PAGE and Western blot, respectively. The anti-viral activity 
      of rPoIFN-gamma was in the range of 450-540 u/mL. In addition, the effect of 
      rPoIFN-gamma ant-PRRSV was determined using CPE50 method. The results indicated 
      that high concentration of rPoIFN-gamma could inhibit PRRSV on Marc-145 cell 
      line. The rPoIFN-gamma is a potential drug for prevention and treatment of 
      various kinds of viral pig diseases.
FAU - Wan, Jian-Qing
AU  - Wan JQ
AD  - College of Veterinary Medicine, Chinese Agricultural University, Beijing 100094, 
      China.
FAU - Wu, Wen-Xue
AU  - Wu WX
FAU - Xia, Chun
AU  - Xia C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Antiviral Agents)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Interferon-gamma/*biosynthesis/*genetics/*pharmacology
MH  - Pichia/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Recombinant Proteins
MH  - Swine
EDAT- 2003/04/05 05:00
MHDA- 2003/07/11 05:00
CRDT- 2003/04/05 05:00
PHST- 2003/04/05 05:00 [pubmed]
PHST- 2003/07/11 05:00 [medline]
PHST- 2003/04/05 05:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2002 Nov;18(6):683-6.