PMID- 12676188
OWN - NLM
STAT- MEDLINE
DCOM- 20030709
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 108
IP  - 5-6
DP  - 2002 Dec 15
TI  - A whole blood flow cytometric determination of platelet activation by 
      unfractionated and low molecular weight heparin in vitro.
PG  - 291-6
AB  - The influence of unfractionated (Heparin-Natrium) and low-molecular heparin 
      (Fragmin(R)) on platelet activation in whole blood was investigated by FACS 
      analysis in vitro using antibodies against glycoprotein (gp) IIb/IIIa (CD 41), 
      GMP 140 (CD 62P), gp 53 (CD 63) and fibrinogen. Samples were also labeled with 
      anti-gp Ib (CD 42b). Neither unfractionated heparin (UFH) nor low molecular 
      weight heparin (LMWH) led to significant (i.e., p<0.05) changes in fluorescence 
      intensities of platelets labeled with anti-gp IIb/IIIa or anti-gp 53. Significant 
      platelet activation due to unfractionated heparin could be observed by labeling 
      with anti-GMP 140 (UFH: p=0.009; LMWH: p=0.16). The proportion of platelets with 
      surface-bound fibrinogen was significantly increased (UFH: p=0.00006; LMWH: 
      p=0.008). After incubation with heparins, activation ability of platelets by 
      adenosine diphosphate (ADP) was significantly increased. The potentiating action 
      of unfractionated heparin was larger. Therefore, flow cytometric results of 
      platelet activation in patients receiving heparin should be interpreted 
      carefully.
FAU - Klein, Bernd
AU  - Klein B
AD  - Department of Gynecology and Obstetrics, University Hospital of the University of 
      Technology (RWTH), Pauwelsstrasse 30, 52074, Aachen, Germany. 
      bernd.klein@post.rwth-aachen.de
FAU - Faridi, Andree
AU  - Faridi A
FAU - von Tempelhoff, G F
AU  - von Tempelhoff GF
FAU - Heilmann, Lothar
AU  - Heilmann L
FAU - Mittermayer, Christian
AU  - Mittermayer C
FAU - Rath, Werner
AU  - Rath W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (P-Selectin)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 9001-32-5 (Fibrinogen)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology
MH  - Adult
MH  - *Blood
MH  - Fibrinogen/analysis/drug effects
MH  - *Flow Cytometry
MH  - Heparin/*pharmacology
MH  - Heparin, Low-Molecular-Weight/pharmacology
MH  - Humans
MH  - P-Selectin/analysis/drug effects
MH  - Platelet Activation/*drug effects
EDAT- 2003/04/05 05:00
MHDA- 2003/07/10 05:00
CRDT- 2003/04/05 05:00
PHST- 2003/04/05 05:00 [pubmed]
PHST- 2003/07/10 05:00 [medline]
PHST- 2003/04/05 05:00 [entrez]
AID - S004938480300094X [pii]
AID - 10.1016/s0049-3848(03)00094-x [doi]
PST - ppublish
SO  - Thromb Res. 2002 Dec 15;108(5-6):291-6. doi: 10.1016/s0049-3848(03)00094-x.