PMID- 12679905
OWN - NLM
STAT- MEDLINE
DCOM- 20030729
LR  - 20240324
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 9
IP  - 4
DP  - 2003 Apr
TI  - Tributyrin inhibits human gastric cancer SGC-7901 cell growth by inducing 
      apoptosis and DNA synthesis arrest.
PG  - 660-4
AB  - AIM: To evaluate the effects of tributyrin, a pro-drug of natural butyrate and a 
      neutral short-chain fatty acid triglyceride, on the growth inhibition of human 
      gastric cancer SGC-7901 cell. METHODS: Human gastric cancer SGC-7901 cells were 
      exposed to tributyrin at 0.5, 1, 2, 5, 10 and 50 mmol/L(-1) for 24-72 h. MTT 
      assay was applied to detect the cell proliferation. [(3)H]-TdR uptake was 
      measured to determine DNA synthesis. Apoptotic morphology was observed by 
      electron microscopy and Hoechst-33258 staining. Flow cytometry and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay were 
      performed to detect tributyrin-triggered apoptosis. The expressions of PARP, 
      Bcl-2 and Bax were examined by Western blot assay. RESULTS: Tributyrin could 
      initiate growth inhibition of SGC-7901 cell in a dose- and time-dependent manner. 
      [(3)H]-TdR uptake by SGC-7901 cells was reduced to 33.6 % after 48 h treatment 
      with 2 mmol/L(-1) tributyrin, compared with the control (P<0.05). Apoptotic 
      morphology was detected by TUNEL assay. Flow cytometry revealed that tributyrin 
      could induce apoptosis of SGC-7901 cells in dose-dependent manner. After 48 hours 
      incubation with tributyrin at 2 mmol/L(-1), the level of Bcl-2 protein was 
      lowered, and the level of Bax protein was increased in SGC-7901, accompanied by 
      PARP cleavage. CONCLUSION: Tributyrin could inhibit the growth of gastric cancer 
      cells effectively in vitro by inhibiting DNA synthesis and inducing apoptosis, 
      which was associated with the down-regulated Bcl-2 expression and the 
      up-regulated Bax expression. Therefore, tributyrin might be a promising 
      chemopreventive and chemotherapeutic agent against human gastric carcinogenesis.
FAU - Yan, Jun
AU  - Yan J
AD  - Lab of Molecular and Cellular Oncology, Institute of Biochemistry and Cell 
      Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of 
      Sciences, 320 Yue Yang Road, Shanghai 200031, China.
FAU - Xu, Yong-Hua
AU  - Xu YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Prodrugs)
RN  - 0 (Triglycerides)
RN  - S05LZ624MF (tributyrin)
SB  - IM
MH  - Antineoplastic Agents/toxicity
MH  - Apoptosis/*drug effects
MH  - Cell Division/drug effects
MH  - DNA Replication/*drug effects
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Prodrugs/toxicity
MH  - Stomach Neoplasms/*pathology/ultrastructure
MH  - Triglycerides/*toxicity
MH  - Tumor Cells, Cultured
PMC - PMC4611423
EDAT- 2003/04/08 05:00
MHDA- 2003/07/30 05:00
PMCR- 2003/04/15
CRDT- 2003/04/08 05:00
PHST- 2003/04/08 05:00 [pubmed]
PHST- 2003/07/30 05:00 [medline]
PHST- 2003/04/08 05:00 [entrez]
PHST- 2003/04/15 00:00 [pmc-release]
AID - 10.3748/wjg.v9.i4.660 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2003 Apr;9(4):660-4. doi: 10.3748/wjg.v9.i4.660.