PMID- 12680458
OWN - NLM
STAT- MEDLINE
DCOM- 20031020
LR  - 20191025
IS  - 0914-9465 (Print)
IS  - 0914-9465 (Linking)
VI  - 65
IP  - 5
DP  - 2002 Dec
TI  - Imaging of chromosomes at nano-meter scale resolution using scanning near-field 
      optical/atomic force microscopy.
PG  - 425-34
AB  - Topographic and fluorescent images of whole barley chromosomes stained with 
      YOYO-1 were observed simultaneously by scanning near-field optical/ atomic force 
      microscopy (SNOM/AFM). The chromosome was relatively smooth and flat in the 
      topographic images and no significant difference in height was present between 
      regions of high fluorescent and low fluorescent intensity in the chromosomes. The 
      telomeric region, labeled by fluorescence in situ hybridization (FISH) method, 
      was also observed by SNOM/AFM at high resolution, and fluorescent signals of the 
      telomeric region were clearly defined on the topographic image of chromatin 
      fibers on the chromosome at the nano-meter scale level. Although the telomeric 
      signals were usually visualized as a single fluorescent region at the end of 
      sister chromatids by conventional light microscopy, they were observed separately 
      as two fluorescent regions, less than 100-200 nm distance, using the SNOM/AFM. 
      The SNOM/AFM offers great potential in identifying particular single gene 
      location on chromosomes in the near future.
FAU - Ohtani, Toshio
AU  - Ohtani T
AD  - National Food Research Institute, Tsukuba, Ibaraki, Japan. ohtani@affrc.go.jp
FAU - Shichirii, Motoharu
AU  - Shichirii M
FAU - Fukushi, Daisuke
AU  - Fukushi D
FAU - Sugiyama, Shigeru
AU  - Sugiyama S
FAU - Yoshino, Tomoyuki
AU  - Yoshino T
FAU - Kobori, Toshiro
AU  - Kobori T
FAU - Hagiwara, Shoji
AU  - Hagiwara S
FAU - Ushiki, Tatsuo
AU  - Ushiki T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Arch Histol Cytol
JT  - Archives of histology and cytology
JID - 8806082
RN  - 0 (Benzoxazoles)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Quinolinium Compounds)
RN  - 143413-85-8 
      (1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium)
SB  - IM
MH  - Benzoxazoles
MH  - Chromosome Banding
MH  - Chromosomes, Plant/*ultrastructure
MH  - Fluorescent Dyes
MH  - Hordeum/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Microscopy, Atomic Force/*methods
MH  - Nanotechnology/*methods
MH  - Quinolinium Compounds
MH  - Telomere/ultrastructure
EDAT- 2003/04/12 05:00
MHDA- 2003/10/21 05:00
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/10/21 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
AID - 10.1679/aohc.65.425 [doi]
PST - ppublish
SO  - Arch Histol Cytol. 2002 Dec;65(5):425-34. doi: 10.1679/aohc.65.425.