PMID- 12680637
OWN - NLM
STAT- MEDLINE
DCOM- 20030530
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 14
IP  - 1
DP  - 2002 Jan
TI  - A comparison of virus isolation, immunohistochemistry, fetal serology, and 
      reverse-transcription polymerase chain reaction assay for the identification of 
      porcine reproductive and respiratory syndrome virus transplacental infection in 
      the fetus.
PG  - 8-14
AB  - Virus isolation (VI), immunohistochemistry (IHC), fetal serology, and 
      reverse-transcription polymerase chain reaction assay (RT-PCR) were performed on 
      samples from 107 fetuses comprising 10 litters taken from sows experimentally 
      infected with porcine reproductive and respiratory syndrome virus (PRRSV). In 
      addition to comparing the relative sensitivity and specificity of each test, 
      RT-PCR was evaluated with respect to the relative suitability of thoracic fluids 
      and tissues as samples, the effects of autolysis, and the effects of pooling of 
      fetal specimens. VI, IHC, and fetal serology identified PRRSV infection in 48.6%, 
      23.4%, and 14.9% of 107 fetuses, respectively, and identified at least 1 infected 
      fetus in 10, 10, and 5 of 10 litters, respectively. In utero death with autolysis 
      reduced the test efficacy of all 3 methods. Fetal thoracic fluid and tissues were 
      equally suitable for RT-PCR detection of PRRSV. Pooling fetal tissues or fluids 
      from VI-positive animals with comparable material from negative controls had no 
      detrimental effect on RT-PCR results when evaluated at dilutions of 1:1, 1:2, 
      1:4, and 1:8. The results of RT-PCR testing were positive in 100%, 94.4%, and 
      83.3% of VI-positive specimens allowed to autolyze at 4, 21, or 37 C, 
      respectively, for 24, 48, and 96 hours. Compared with the other testing 
      modalities, RT-PCR appeared to be impacted the least by the adverse effects of 
      autolysis.
FAU - Benson, James E
AU  - Benson JE
AD  - Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA 50011, USA.
FAU - Yaeger, Michael J
AU  - Yaeger MJ
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
FAU - Lager, Kelly
AU  - Lager K
FAU - Yoon, Kyoung-Jin
AU  - Yoon KJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
SB  - IM
MH  - Animals
MH  - Female
MH  - Fetal Diseases/immunology/*veterinary/*virology
MH  - Fetus/immunology/*virology
MH  - Immunohistochemistry
MH  - *Infectious Disease Transmission, Vertical
MH  - Porcine Reproductive and Respiratory 
      Syndrome/diagnosis/immunology/*transmission/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/immunology/veterinary/virology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Swine Diseases/immunology/*transmission/*virology
EDAT- 2003/04/12 05:00
MHDA- 2003/05/31 05:00
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/05/31 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
AID - 10.1177/104063870201400103 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2002 Jan;14(1):8-14. doi: 10.1177/104063870201400103.