PMID- 12680638
OWN - NLM
STAT- MEDLINE
DCOM- 20030530
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 14
IP  - 1
DP  - 2002 Jan
TI  - The diagnostic sensitivity of immunohistochemistry for the detection of porcine 
      reproductive and respiratory syndrome virus in the lung of vaccinated and 
      unvaccinated swine.
PG  - 15-9
AB  - Immunohistochemistry (IHC) is used routinely to detect porcine reproductive and 
      respiratory syndrome virus (PRRSV) in the lung of nursery and grow/finish pigs 
      with respiratory disease and has been reported to be highly specific (100%) but 
      only moderately sensitive (67%). When multiple sections of lung are examined from 
      field cases of porcine pneumonia, it is common to detect PRRSV antigen in only 1 
      or 2 of the sections. This study was undertaken to determine the impact of the 
      number of lung sections evaluated on the diagnostic sensitivity of IHC for the 
      detection of PRRSV in vaccinated and unvaccinated swine. Five anterioventral 
      sections of lung from animals experimentally challenged with PRRSV were evaluated 
      on a single IHC slide. Utilizing a beta binomial model, observed results were 
      used to calculate the probability of detecting PRRSV with IHC as a function of 
      the number of lung sections assessed. Results demonstrate that the diagnostic 
      sensitivity of PRRSV IHC is dependent on the number of lung sections examined. In 
      unvaccinated pigs, a beta binomial model predicts that if a single lung section 
      were evaluated, PRRSV would likely be confirmed in only 48% of infected animals, 
      and at least 5 sections of anterioventral lung would need to be assessed to 
      detect >90% of PRRSV-infected pigs. Vaccination resulted in significantly lower 
      gross and microscopic lung lesion scores and significantly fewer antigen-positive 
      cells. In vaccinated swine, the calculated probability of detecting a 
      PRRSV-infected pig with IHC when a single lung section is evaluated was only 14%. 
      If PRRSV is a primary concern, diagnosticians should collect at least 5 
      anterioventral sections of lung from each pig to be evaluated on a single IHC 
      slide. This approach will diminish the number of false-negative results obtained 
      with this method of antigen detection.
FAU - Yaeger, Michael J
AU  - Yaeger MJ
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Immunohistochemistry
MH  - Lung/pathology/*virology
MH  - Porcine Reproductive and Respiratory 
      Syndrome/*diagnosis/*immunology/pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/*isolation & 
      purification
MH  - Sensitivity and Specificity
MH  - Swine/immunology/*virology
MH  - Vaccination
MH  - Viral Vaccines/*immunology
EDAT- 2003/04/12 05:00
MHDA- 2003/05/31 05:00
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/05/31 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
AID - 10.1177/104063870201400104 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2002 Jan;14(1):15-9. doi: 10.1177/104063870201400104.