PMID- 12680645
OWN - NLM
STAT- MEDLINE
DCOM- 20030530
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 14
IP  - 1
DP  - 2002 Jan
TI  - Detection and differentiation of North American and European genotypes of porcine 
      reproductive and respiratory syndrome virus in formalin-fixed, paraffin-embedded 
      tissues by multiplex reverse transcription-nested polymerase chain reaction.
PG  - 56-60
AB  - North American and European genotypes of porcine reproductive and respiratory 
      syndrome virus (PRRSV) were distinguished in formalin-fixed, paraffin-embedded 
      tissues from PRRSV-infected pigs by multiplex reverse transcription-nested 
      polymerase chain reaction (RT-nPCR). A method based on xylene deparaffinization 
      followed by proteinase K digestion yielded RNA of quality for reliable and 
      consistent RT-nPCR analyses. The PRRSV nucleic acid was detected in lung, 
      mediastinal lymph node, tonsil, and liver samples from pigs inoculated with North 
      American strain virus, European strain virus, or both North American and European 
      strains. All 30 archival formalin-fixed, paraffin-embedded lung tissues from pigs 
      naturally infected with PRRSV were positive for PRRSV by RT-nPCR amplication and 
      gave a pattern that corresponded to the North American genotype. Multiplex 
      RT-nPCR could be applied successfully to formalin-fixed, paraffin-embedded 
      tissues for the detection and differentiation between North American and European 
      genotypes of PRRSV.
FAU - Chung, Han-Kook
AU  - Chung HK
AD  - Department of Veterinary Pathology, College of Veterinary Medicine and School of 
      Agricultural Biotechnology, Seoul National University, Suwon 441-744, 
      Kyounggi-Do, Republic of Korea.
FAU - Choi, Changsun
AU  - Choi C
FAU - Kim, Junghyun
AU  - Kim J
FAU - Chae, Chanhee
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (DNA, Viral)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Animals
MH  - DNA, Viral/*analysis/genetics
MH  - Europe
MH  - Formaldehyde
MH  - Genotype
MH  - Liver/virology
MH  - Lung/virology
MH  - Lymph Nodes/virology
MH  - North America
MH  - Palatine Tonsil/virology
MH  - *Paraffin Embedding
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/*isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Swine/*virology
MH  - *Tissue Fixation
EDAT- 2003/04/12 05:00
MHDA- 2003/05/31 05:00
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/05/31 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
AID - 10.1177/104063870201400111 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2002 Jan;14(1):56-60. doi: 10.1177/104063870201400111.