PMID- 12680875
OWN - NLM
STAT- MEDLINE
DCOM- 20030513
LR  - 20220316
IS  - 0960-7722 (Print)
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Linking)
VI  - 36
IP  - 2
DP  - 2003 Apr
TI  - Stromal cell-derived factor 1alpha (SDF-1alpha) induces gene-expression of early 
      growth response-1 (Egr-1) and VEGF in human arterial endothelial cells and 
      enhances VEGF induced cell proliferation.
PG  - 75-86
AB  - Stromal cell-derived factor-1 (SDF-1), mainly known as a chemotactic factor for 
      haematopoietic progenitor cells, also provides angiogenetic potency. Since the 
      intracellular signalling of SDF-1-induced neovascularization remains unclear, we 
      studied in human umbilical arterial endothelial cells (HUAEC) the influence of 
      SDF-1alpha on induction of the genes of early growth response-1 (Egr-1) and VEGF, 
      as well as the activation of extracellular regulated kinases (ERK) 1/2, which are 
      all known to be involved in endothelial cell proliferation. We found a 
      time-dependent induction of Egr-1 and VEGF mRNA expression and phosphorylation of 
      ERK1/2 by SDF-1alpha. Furthermore, we demonstrated that Egr-1 expression is 
      dependent on ERK 1/2 activation. Finally, we tried to confirm the relevance of 
      the induced gene expression by detecting the [3H]thymidine incorporation as a 
      marker for cell proliferation in HUAEC after stimulation with SDF-1alpha alone or 
      together with VEGF. This particular test showed, that SDF-1alpha alone has no 
      effect, but is able to significantly enhance VEGF induced DNA synthesis. In 
      summary, SDF-1alpha is involved in different steps of endothelial cell 
      proliferation, but, since Egr-1 and VEGF offer different functions, it may also 
      play a so far undefined role on other conditions of the endothelium.
FAU - Neuhaus, Thomas
AU  - Neuhaus T
AD  - Medizinische Universitats-Poliklinik Bonn, Bonn, Germany.
FAU - Stier, Sebastian
AU  - Stier S
FAU - Totzke, Gudrun
AU  - Totzke G
FAU - Gruenewald, Elisabeth
AU  - Gruenewald E
FAU - Fronhoffs, Stefan
AU  - Fronhoffs S
FAU - Sachinidis, Agapios
AU  - Sachinidis A
FAU - Vetter, Hans
AU  - Vetter H
FAU - Ko, Yon D
AU  - Ko YD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EGR1 protein, human)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lymphokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Arteries/cytology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/*pharmacology
MH  - DNA/biosynthesis
MH  - DNA-Binding Proteins/*biosynthesis/genetics
MH  - Drug Synergism
MH  - Early Growth Response Protein 1
MH  - Endothelial Growth Factors/*biosynthesis/*pharmacology/physiology
MH  - Endothelium, Vascular/drug effects/enzymology/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation
MH  - Humans
MH  - *Immediate-Early Proteins
MH  - Intercellular Signaling Peptides and 
      Proteins/*biosynthesis/*pharmacology/physiology
MH  - Kinetics
MH  - Lymphokines/*biosynthesis/*pharmacology/physiology
MH  - Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Phosphorylation
MH  - RNA, Messenger/biosynthesis
MH  - Transcription Factors/*biosynthesis/genetics
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
MH  - p38 Mitogen-Activated Protein Kinases
PMC - PMC6496392
EDAT- 2003/04/12 05:00
MHDA- 2003/05/14 05:00
PMCR- 2003/04/04
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/05/14 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
PHST- 2003/04/04 00:00 [pmc-release]
AID - 262 [pii]
AID - CPR262 [pii]
AID - 10.1046/j.1365-2184.2003.00262.x [doi]
PST - ppublish
SO  - Cell Prolif. 2003 Apr;36(2):75-86. doi: 10.1046/j.1365-2184.2003.00262.x.