PMID- 12682160
OWN - NLM
STAT- MEDLINE
DCOM- 20030711
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 41
IP  - 4
DP  - 2003 Apr
TI  - Clinical value of specific immunoglobulin E detection by enzyme-linked 
      immunosorbent assay in cases of acquired and congenital toxoplasmosis.
PG  - 1681-6
AB  - The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) 
      detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 
      2,036 sera from 792 subjects, comprising seronegative controls and subjects with 
      acute, active, reactivated, or congenital toxoplasmosis. Included were 
      nonimmunized adults; pregnant women with recently acquired infection (acute 
      toxoplasmosis); immunocompetent subjects with recently acquired severe infection 
      (active toxoplasmosis) expressed as fever, adenopathies, splenomegaly, pneumonia, 
      meningitis, or disseminated infection; subjects-some of them 
      immunocompromised-whose previously moderate IgG antibody levels rose, suggesting 
      a reactivation of quiescent toxoplasmosis; and infants born to seroconverted 
      mothers and evaluated for diagnosis of congenital infection and therapeutic 
      management. Specific IgE antibodies were never detected in seronegative subjects. 
      They were present in 85.7% of asymptomatic seroconverters and in 100% of 
      seroconverters with overt toxoplasmosis, following two different kinetics: in the 
      former, the specific IgE titer generally presented a brief peak 2 to 3 months 
      postinfection and then fell rapidly, whereas specific IgE persisted at a very 
      high titer for several months in the latter. IgE emerged concomitantly with the 
      increase in IgG during toxoplasmic reactivation. For neonatal diagnosis of 
      congenital toxoplasmosis, IgE was less informative than IgM and IgA 
      (sensitivities, 59.5, 64.3, and 76.2%, respectively) and had a specificity of 
      91.9%. Nevertheless, simultaneous measurement of the three isotypes at birth 
      improved the diagnostic yield to 81% relative to the combination of IgA and IgM. 
      Emergence of specific IgE during postnatal treatment for congenital toxoplasmosis 
      is a sign of poor adherence or inadequate dosing.
FAU - Foudrinier, F
AU  - Foudrinier F
AD  - Toxoplasmosis Group, Laboratory of Parasitology-Mycology, IFR53, EA 2070, Hopital 
      Maison Blanche, 51092 Reims, France. ffoudrinier@chu-reims.fr
FAU - Villena, I
AU  - Villena I
FAU - Jaussaud, R
AU  - Jaussaud R
FAU - Aubert, D
AU  - Aubert D
FAU - Chemla, C
AU  - Chemla C
FAU - Martinot, F
AU  - Martinot F
FAU - Pinon, J M
AU  - Pinon JM
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Protozoan)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Blood/immunology
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Infant
MH  - Male
MH  - Pregnancy
MH  - Pregnancy Complications, Parasitic/*diagnosis/immunology
MH  - Toxoplasma/immunology
MH  - Toxoplasmosis/*diagnosis/immunology/parasitology
MH  - Toxoplasmosis, Congenital/*diagnosis/immunology/parasitology
PMC - PMC153890
EDAT- 2003/04/12 05:00
MHDA- 2003/07/12 05:00
PMCR- 2003/04/01
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/07/12 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
PHST- 2003/04/01 00:00 [pmc-release]
AID - 1191 [pii]
AID - 10.1128/JCM.41.4.1681-1686.2003 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2003 Apr;41(4):1681-6. doi: 10.1128/JCM.41.4.1681-1686.2003.