PMID- 12682240
OWN - NLM
STAT- MEDLINE
DCOM- 20030731
LR  - 20201212
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 170
IP  - 8
DP  - 2003 Apr 15
TI  - A subset of Toll-like receptor ligands induces cross-presentation by bone 
      marrow-derived dendritic cells.
PG  - 4102-10
AB  - Dendritic cells (DCs) are capable of cross-presenting exogenous Ag to CD8(+) 
      CTLs. Detection of microbial products by Toll-like receptors (TLRs) leads to 
      activation of DCs and subsequent orchestration of an adaptive immune response. We 
      hypothesized that microbial TLR ligands could activate DCs to cross-present Ag to 
      CTLs. Using DCs and CTLs in an in vitro cross-presentation system, we show that a 
      subset of microbial TLR ligands, namely ligands of TLR3 (poly(inosinic-cytidylic) 
      acid) and TLR9 (immunostimulatory CpG DNA), induces cross-presentation. In 
      contrast to presentation of Ag to CD4(+) T cells by immature DCs, TLR-induced 
      cross-presentation is mediated by mature DCs, is independent of endosomal 
      acidification, and relies on cytosolic Ag processing machinery.
FAU - Datta, Sandip K
AU  - Datta SK
AD  - Department of Medicine and The Sam and Rose Stein Institute for Research on 
      Aging, University of California, La Jolla, CA 92093, USA.
FAU - Redecke, Vanessa
AU  - Redecke V
FAU - Prilliman, Kiley R
AU  - Prilliman KR
FAU - Takabayashi, Kenji
AU  - Takabayashi K
FAU - Corr, Maripat
AU  - Corr M
FAU - Tallant, Thomas
AU  - Tallant T
FAU - DiDonato, Joseph
AU  - DiDonato J
FAU - Dziarski, Roman
AU  - Dziarski R
FAU - Akira, Shizuo
AU  - Akira S
FAU - Schoenberger, Stephen P
AU  - Schoenberger SP
FAU - Raz, Eyal
AU  - Raz E
LA  - eng
GR  - AI07036-22/AI/NIAID NIH HHS/United States
GR  - AI40682/AI/NIAID NIH HHS/United States
GR  - AI47078/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Peptidoglycan)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (Toll-Like Receptors)
RN  - 12777-81-0 (Flagellin)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Adjuvants, Immunologic/physiology
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, Bacterial/immunology/metabolism
MH  - Antigens, Differentiation/physiology
MH  - Bone Marrow Cells/cytology/*immunology/*metabolism
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - CpG Islands/immunology
MH  - Cytosol/immunology/metabolism
MH  - Dendritic Cells/cytology/*immunology/*metabolism
MH  - Down-Regulation/immunology
MH  - Flagellin/immunology/metabolism
MH  - Histocompatibility Antigens Class I/immunology/metabolism
MH  - Histocompatibility Antigens Class II/immunology/metabolism
MH  - Intracellular Fluid/immunology/metabolism
MH  - Ligands
MH  - Lipopolysaccharides/immunology/metabolism
MH  - Male
MH  - Membrane Glycoproteins/biosynthesis/*metabolism/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Myeloid Differentiation Factor 88
MH  - Peptidoglycan/immunology/metabolism
MH  - Poly I-C/immunology/metabolism
MH  - Receptors, Cell Surface/biosynthesis/*metabolism/physiology
MH  - Receptors, Immunologic/physiology
MH  - Toll-Like Receptor 3
MH  - Toll-Like Receptors
EDAT- 2003/04/19 05:00
MHDA- 2003/08/02 05:00
CRDT- 2003/04/19 05:00
PHST- 2003/04/19 05:00 [pubmed]
PHST- 2003/08/02 05:00 [medline]
PHST- 2003/04/19 05:00 [entrez]
AID - 10.4049/jimmunol.170.8.4102 [doi]
PST - ppublish
SO  - J Immunol. 2003 Apr 15;170(8):4102-10. doi: 10.4049/jimmunol.170.8.4102.