PMID- 12682250
OWN - NLM
STAT- MEDLINE
DCOM- 20030731
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 170
IP  - 8
DP  - 2003 Apr 15
TI  - Regulation of MHC class I transport in human dendritic cells and the 
      dendritic-like cell line KG-1.
PG  - 4178-88
AB  - Dendritic cells (DCs) progress through distinct maturational phases; immature DCs 
      capture Ag while mature DCs are optimized for Ag presentation. Proper control of 
      immunity requires regulated compartmentalization of MHC class II molecules. We 
      report that DCs also regulate MHC class I trafficking throughout maturation. 
      Although mature human DCs express high levels of surface MHC class I, immature 
      DCs exhibit lower surface levels while retaining MHC class I-peptide complexes in 
      the Golgi. A cell line, KG-1, behaves similarly. We confirm the similarity of 
      KG-1 to DCs by demonstrating its capacity to present exogenous Ags in an MHC 
      class I-restricted fashion to CD8(+) T cell hybridomas, a phenomenon called 
      cross-presentation. Biochemical characterization of MHC class I trafficking 
      throughout maturation showed that, in early KG-1 dendritic-like cells, surface 
      arrival of MHC class I-peptide complexes is delayed by their retention in the 
      Golgi. In mature dendritic-like cells, these complexes relocate to the surface 
      and their stability increases, concomitant with up-regulation of costimulatory 
      molecules. Maturation induces qualitative changes in the MHC class I-associated 
      peptide repertoire demonstrated by increased thermostability. The differential 
      processing of MHC class I throughout maturation may prevent premature immune 
      activation while promoting T cell responses in lymph nodes to Ags acquired at 
      sites of inflammation.
FAU - Ackerman, Anne L
AU  - Ackerman AL
AD  - Section of Immunobiology, Howard Hughes Medical Institute, Yale University School 
      of Medicine, New Haven, CT 06520, USA.
FAU - Cresswell, Peter
AU  - Cresswell P
LA  - eng
GR  - AI10347-02/AI/NIAID NIH HHS/United States
GR  - AI23081/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Egg Proteins)
RN  - 0 (H-2 Antigens)
RN  - 0 (H-2Kb protein, mouse)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (OVA-8)
RN  - 0 (Peptide Fragments)
RN  - 0 (Peptides)
RN  - 0 (beta 2-Microglobulin)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigen-Presenting Cells/cytology/immunology/metabolism
MH  - Cell Differentiation/immunology
MH  - Dendritic Cells/cytology/*immunology/*metabolism
MH  - Dimerization
MH  - Egg Proteins/immunology/metabolism
MH  - H-2 Antigens/immunology/metabolism
MH  - HLA Antigens/biosynthesis/metabolism
MH  - Histocompatibility Antigens Class I/biosynthesis/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Models, Biological
MH  - Ovalbumin/immunology/metabolism
MH  - Peptide Fragments
MH  - Peptides/immunology/metabolism
MH  - Protein Binding/immunology
MH  - Protein Transport/immunology
MH  - Tumor Cells, Cultured/*immunology/*metabolism
MH  - beta 2-Microglobulin/biosynthesis/metabolism
EDAT- 2003/04/12 05:00
MHDA- 2003/08/02 05:00
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/08/02 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
AID - 10.4049/jimmunol.170.8.4178 [doi]
PST - ppublish
SO  - J Immunol. 2003 Apr 15;170(8):4178-88. doi: 10.4049/jimmunol.170.8.4178.