PMID- 12682631
OWN - NLM
STAT- MEDLINE
DCOM- 20030505
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 17
IP  - 4
DP  - 2003 Apr
TI  - Molecular cytogenetic detection of chromosomal breakpoints in T-cell receptor 
      gene loci.
PG  - 738-45
AB  - Chromosomal aberrations with breakpoints in T-cell receptor (TCR) gene loci are 
      recurrent in several T-cell malignancies. Although the importance of interphase 
      cytogenetics has been extensively shown in B-cell lymphomas, hardly any molecular 
      cytogenetic tools are available for recurrent changes in T-cell disorders. Thus, 
      we have established fluorescence in situ hybridization (FISH)-based break-apart 
      assays for the TCRA/D (14q11), TCRB (7q34) and TCRG (7p14) genes and the TCL 
      cluster (14q32). The assays were validated in normal controls as well as in 43 
      T-cell malignancies with cytogenetically proven 14q11, 7q34-35 or 7p13-21 
      aberrations. Breakpoints in TCRA/D, TCRB and TCRG could be diagnosed by these 
      assays in 32/33 T-cell neoplasms with chromosome 14q11, 3/6 with 7q34-35 and 1/7 
      with 7p13-21 alterations, respectively. Application of the new FISH assays to a 
      series of 24 angioimmunoblastic and 12 cutaneous T-cell lymphomas confirmed the 
      cytogenetic evidence of lack of breakpoints in the TCRA/D or TCRB locus. 
      Simultaneous detection of TCRA/D or TCRB breaks was achieved in a multicolor 
      approach, which was further combined with detection of the T-cell-specific CD3 
      antigen in a multicolor FICTION (Fluorescence Immunophenotyping and Interphase 
      Cytogenetics as a Tool for the Investigation of Neoplasm) assay. These new FISH 
      and FICTION assays provide sensitive, rapid and accurate tools for the diagnosis 
      and biological characterization of T-cell malignancies.
FAU - Gesk, S
AU  - Gesk S
AD  - Institute of Human Genetics, University Hospital Schleswig-Holstein, Kiel, 
      Germany.
FAU - Martin-Subero, J I
AU  - Martin-Subero JI
FAU - Harder, L
AU  - Harder L
FAU - Luhmann, B
AU  - Luhmann B
FAU - Schlegelberger, B
AU  - Schlegelberger B
FAU - Calasanz, M J
AU  - Calasanz MJ
FAU - Grote, W
AU  - Grote W
FAU - Siebert, R
AU  - Siebert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child, Preschool
MH  - *Chromosome Breakage
MH  - Chromosome Inversion
MH  - Chromosome Painting/methods
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - False Positive Reactions
MH  - Female
MH  - Humans
MH  - Karyotyping
MH  - Leukemia, T-Cell/*genetics
MH  - Lymphoma, T-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Mycosis Fungoides/genetics
MH  - Receptors, Antigen, T-Cell, alpha-beta/*genetics
MH  - Receptors, Antigen, T-Cell, gamma-delta/*genetics
MH  - Sequence Deletion
MH  - Sezary Syndrome/genetics
MH  - Translocation, Genetic
EDAT- 2003/04/12 05:00
MHDA- 2003/05/06 05:00
CRDT- 2003/04/12 05:00
PHST- 2003/04/12 05:00 [pubmed]
PHST- 2003/05/06 05:00 [medline]
PHST- 2003/04/12 05:00 [entrez]
AID - 2402884 [pii]
AID - 10.1038/sj.leu.2402884 [doi]
PST - ppublish
SO  - Leukemia. 2003 Apr;17(4):738-45. doi: 10.1038/sj.leu.2402884.