PMID- 12684937
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 19
IP  - 2
DP  - 1990
TI  - Effect of immune-mediated erythrocyte agglutination on analysis of canine blood 
      using a multichannel blood cell counting system.
PG  - 45-50
AB  - Blood from six dogs with in vitro immune-mediated erythrocyte agglutination 
      resulted in analytical errors in directly measured counting and sizing functions 
      on a multichannel blood analysis system with histogram capability. Errors in the 
      directly measured values, mean cell volume (MCV), and erythrocyte count were 
      attributed to agglutinated erythrocyte particles that persisted during the 
      relatively short reagent contact time of the analysis. Agglutinated particles 
      less than 240 fl were visible on erythrocyte histograms and resulted in a false 
      low erythrocyte count and false high MCV. Agglutinated cell particles greater 
      than 240 fl were not present on the histogram scale. Because these latter 
      particles exceeded the upper threshold, they did not influence determination of 
      MCV, but resulted in a further decrease in the erythrocyte count. As a result, 
      the other dependent erythrocyte indices were in error. These included false low 
      hematocrit and false high mean corpuscular hemoglobin concentration (MCHC), when 
      compared to corrected reference blood values. Similar errors occurred when 
      analyzing blood samples that were agglutinated in vitro by incubating 
      erythrocytes with incompatible plasma. The counting and sizing errors observed 
      with electronic counting techniques were eliminated or greatly reduced by 
      incubating blood in cell counting diluent for 10 minutes followed by analysis on 
      a single channel counter with attached particle size analyzer. Error in 
      erythrocyte measurement on a multichannel system may be anticipated if there is 
      overt erythrocyte agglutination in a blood sample, an abnormally high MCHC is 
      reported by the system, or subpopulations of large volume (agglutinated cells) 
      are observed on a volume distribution histogram.
FAU - Porter, Robert E Jr
AU  - Porter RE Jr
AD  - Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, 
      Colorado Sate University, Fort Collins, CO 80523.
FAU - Weiser, M Glade
AU  - Weiser MG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
EDAT- 1990/01/01 00:00
MHDA- 2003/04/10 05:00
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 2003/04/10 05:00 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1111/j.1939-165x.1990.tb00542.x [doi]
PST - ppublish
SO  - Vet Clin Pathol. 1990;19(2):45-50. doi: 10.1111/j.1939-165x.1990.tb00542.x.