PMID- 12686761
OWN - NLM
STAT- MEDLINE
DCOM- 20030508
LR  - 20190910
IS  - 1347-8613 (Print)
IS  - 1347-8613 (Linking)
VI  - 91
IP  - 2
DP  - 2003 Feb
TI  - Augmentation by Bursaphelenchus xylophilus, a pine wood nematode, of polyclonal 
      IgE production induced by lipopolysaccharide plus interleukin-4 in murine 
      splenocytes.
PG  - 158-62
AB  - Bursaphelenchus xylophilus (B. xylophilus) is a pine wood nematode that is known 
      to cause pine wilt disease. We report here that B. xylophilus extracts augmented 
      the polyclonal immunoglobulin E (IgE) production induced by lipopolysaccharide 
      (LPS) plus interleukin-4 (IL-4) both in murine splenocytes and purified B cells 
      as determined by ELISA and ELIspot assays, but they did not cause such a 
      promotion in the absence of either LPS or IL-4. We also observed that the 
      antigen-nonspecific IgE levels were increased in sera of mice treated with B. 
      xylophilus extracts, which were comparable to those of Ascaris suum extracts. 
      These findings suggest that administration of B. xylophilus extracts could 
      suppress allergic diseases via a saturation of mast cell Fcepsilon receptors 
      or/and an inhibition of antigen-specific IgE synthesis to the allergen by a 
      polyclonal response.
FAU - Kaji, Hiroaki
AU  - Kaji H
AD  - Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama 
      University, Okayama, Japan.
FAU - Kawada, Masahiko
AU  - Kawada M
FAU - Tai, Akihiro
AU  - Tai A
FAU - Kanzaki, Hiroshi
AU  - Kanzaki H
FAU - Yamamoto, Itaru
AU  - Yamamoto I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - J Pharmacol Sci
JT  - Journal of pharmacological sciences
JID - 101167001
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Fc)
RN  - 0 (Tissue Extracts)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Animals
MH  - Ascaris suum/chemistry
MH  - B-Lymphocytes/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Immunoglobulin E/*biosynthesis
MH  - In Vitro Techniques
MH  - Interleukin-4/*pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - Mast Cells/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nematoda/*physiology
MH  - Receptors, Fc/antagonists & inhibitors
MH  - Spleen/cytology/drug effects/*metabolism
MH  - Stimulation, Chemical
MH  - Tissue Extracts/*pharmacology
EDAT- 2003/04/11 05:00
MHDA- 2003/05/09 05:00
CRDT- 2003/04/11 05:00
PHST- 2003/04/11 05:00 [pubmed]
PHST- 2003/05/09 05:00 [medline]
PHST- 2003/04/11 05:00 [entrez]
AID - 10.1254/jphs.91.158 [doi]
PST - ppublish
SO  - J Pharmacol Sci. 2003 Feb;91(2):158-62. doi: 10.1254/jphs.91.158.