PMID- 12691906
OWN - NLM
STAT- MEDLINE
DCOM- 20030807
LR  - 20131121
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 37
IP  - 9
DP  - 2003 May
TI  - Evaluation of the impact of bioaugmentation and biostimulation by in situ 
      hybridization and microelectrode.
PG  - 2206-16
AB  - Three rotating disk biofilm reactors were operated to evaluate whether 
      bioaugmentation and biostimulation can be used to improve the start-up of 
      microbial nitrification. The first reactor was bioaugmented during start-up 
      period with an enrichment culture of nitrifying bacteria, the second reactor 
      received a synthetic medium containing NH(4)(+) and NO(2)(-) to facilitate 
      concomitant proliferation of ammonia- and nitrite-oxidizing bacteria, and the 
      third reactor was used as a control. To evaluate the effectiveness of 
      bioaugmentation and biostimulation approaches, time-dependent developments of 
      nitrifying bacterial community and in situ nitrifying activity in biofilms were 
      monitored by fluorescence in situ hybridization (FISH) technique and 
      microelectrode measurements of NH(4)(+), NO(2)(-), NO(3)(-), and O(2). In situ 
      hybridization results revealed that addition of the enrichment culture of 
      nitrifying bacteria significantly facilitated development of dense nitrifying 
      bacterial populations in the biofilm shortly after, which led to a rapid start-up 
      and enhancement of in situ nitrification activity. The inoculated bacteria could 
      proliferate and/or survive in the biofilm. In addition, the addition of 
      nitrifying bacteria increased the abundance of nitrifying bacteria in the surface 
      of the biofilm, resulting in the higher nitrification rate. On the other hand, 
      the addition of 2.1mM NO(2)(-) did not stimulate the growth of nitrite-oxidizing 
      bacteria and did inhibit the proliferation of ammonia-oxidizing bacteria instead. 
      Thus, the start-up of NO(2)(-) oxidation was unchanged, and the start-up of 
      NH(4)(+) oxidation was delayed. In all the three biofilm reactors, data sets of 
      time series analyses on population dynamics of nitrifying bacteria determined by 
      FISH, in situ nitrifying activities determined by microelectrode measurements, 
      and the reactor performances revealed an approximate agreement between the 
      appearance of nitrifying bacteria and the initiation of nitrification activity, 
      suggesting that the combination of these techniques was a very powerful 
      monitoring tool to evaluate the effectiveness of bioaugmentation and 
      biostimulation strategies.
FAU - Satoh, Hisashi
AU  - Satoh H
AD  - Department of Environmental and Civil Engineering, Hachinohe Institute of 
      Technology, Aomori 031-8501, Japan.
FAU - Okabe, Satoshi
AU  - Okabe S
FAU - Yamaguchi, Yuki
AU  - Yamaguchi Y
FAU - Watanabe, Yoshimasa
AU  - Watanabe Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - *Biofilms
MH  - *Bioreactors
MH  - DNA Primers
MH  - DNA, Bacterial/analysis
MH  - Electrodes
MH  - In Situ Hybridization, Fluorescence
MH  - *Models, Theoretical
MH  - Nitrogen/*metabolism
MH  - Oxidation-Reduction
EDAT- 2003/04/15 05:00
MHDA- 2003/08/09 05:00
CRDT- 2003/04/15 05:00
PHST- 2003/04/15 05:00 [pubmed]
PHST- 2003/08/09 05:00 [medline]
PHST- 2003/04/15 05:00 [entrez]
AID - S0043-1354(02)00617-6 [pii]
AID - 10.1016/S0043-1354(02)00617-6 [doi]
PST - ppublish
SO  - Water Res. 2003 May;37(9):2206-16. doi: 10.1016/S0043-1354(02)00617-6.