PMID- 12694377
OWN - NLM
STAT- MEDLINE
DCOM- 20030807
LR  - 20190922
IS  - 0953-8194 (Print)
IS  - 0953-8194 (Linking)
VI  - 15
IP  - 5
DP  - 2003 May
TI  - Stimulation of luteinizing hormone secretion by N-methyl-D,L-aspartic acid in the 
      adult male guinea-pig: incomplete blockade by gonadotropin-releasing hormone 
      receptor antagonism.
PG  - 516-20
AB  - Stimulation of luteinizing hormone (LH) secretion by N-methyl-D,L-aspartic acid 
      (NMA), reported for several mammalian species, is generally accepted to be 
      mediated through stimulation of hypothalamic gonadotropin-releasing hormone 
      (GnRH) release. In view of a previously reported unexpected inhibitory action of 
      NMA on GnRH release from hypothalamic explant of intact male guinea-pigs, the aim 
      of the present study was to assess the in vivo effects of NMA in the adult male 
      guinea-pig. In the gonadally intact male, NMA (5 mg/animal) elicited a robust LH 
      secretion, which was blocked by the N-methyl-D-asparte-receptor antagonist 
      DL-2-amino-5-phosphonovaleric acid (AP-5,12 mg/animal). In the castrated male, 
      NMA elicited only a marginal and inconsistent LH secretion. Cetrorelix (CET), a 
      GnRH receptor antagonist, administered intracardiacally 1 min or 45 min preceding 
      bolus injection of NMA significantly reduced the LH response to NMA in the intact 
      male. Surprisingly, following GnRH receptor blockade with CET, there still was a 
      substantial residual serum LH response to NMA, while CET completely abolished the 
      serum LH response to high dose (1 microg or 10 microg) guinea-pig GnRH (gpGnRH). 
      These results indicate that NMA stimulates LH secretion in the gonadally intact 
      male guinea-pig in vivo and that this effect is mediated in part through 
      gpGnRH-independent mechanisms.
FAU - Gao, C-Q
AU  - Gao CQ
AD  - Department of Endocrinology and Heymans Institute of Pharmacology, Ghent 
      University, Ghent, Belgium.
FAU - Kaufman, J-M
AU  - Kaufman JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Receptors, LHRH)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 76326-31-3 (2-amino-5-phosphopentanoic acid)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - HG18B9YRS7 (Valine)
RN  - OON1HFZ4BA (cetrorelix)
SB  - IM
MH  - Animals
MH  - Gonadotropin-Releasing Hormone/analogs & derivatives/pharmacology
MH  - Guinea Pigs
MH  - Luteinizing Hormone/blood/*metabolism
MH  - Male
MH  - N-Methylaspartate/*pharmacology
MH  - Receptors, LHRH/*antagonists & inhibitors/physiology
MH  - Valine/*analogs & derivatives/pharmacology
EDAT- 2003/04/16 05:00
MHDA- 2003/08/09 05:00
CRDT- 2003/04/16 05:00
PHST- 2003/04/16 05:00 [pubmed]
PHST- 2003/08/09 05:00 [medline]
PHST- 2003/04/16 05:00 [entrez]
AID - 1024 [pii]
AID - 10.1046/j.1365-2826.2003.01024.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2003 May;15(5):516-20. doi: 10.1046/j.1365-2826.2003.01024.x.