PMID- 12694545
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20191107
IS  - 0908-665X (Print)
IS  - 0908-665X (Linking)
VI  - 10
IP  - 3
DP  - 2003 May
TI  - Porcine CD80: cloning, characterization, and evidence for its role in direct 
      human T-cell activation.
PG  - 252-8
AB  - Previous studies has shown that human anti-pig reactivity in mixed lymphocyte 
      cultures require the indirect presentation of antigens by human antigen 
      presenting cells (APC). Xenoreactivity was inhibited by blockade of human 
      costimulatory molecules. We investigated the role of porcine costimulatory 
      molecules in their ability to activate human T cells directly. Porcine CD80 was 
      cloned from lipopolysaccharide (LPS)-activated porcine lymphocytes. Sequence 
      analysis showed a high degree of conservation in residues involved in CD28/CTLA4. 
      COS cells transfected with porcine CD80 was able to activate human T cells in a 
      cyclosporine independent manner, demonstrating that porcine CD80 can costimulate 
      human T cells. Tumor necrosis factor-alpha (TNF-alpha) activated porcine 
      splenocytes have been shown to up-regulate B7s. In order to test the effect of 
      costimulation blockade in a xeno system, activated splenocytes were cultured with 
      purified CD4+ T cells. The results demonstrated that these cells were capable of 
      activating human T cells and this activation can be blocked by using an antihuman 
      CD80 antibody that demonstrated cross-reactivity to porcine CD80. Non-cross 
      reactive antibodies had no effect, again suggesting direct activation of the 
      human T cells. These data suggest that a reagent that can block both the direct 
      and indirect activation is necessary for a discordant xenotransplant.
FAU - Tadaki, D K
AU  - Tadaki DK
AD  - NIDDK/Navy Transplantation and Autoimmunity Branch, Naval Medical Research 
      Center, Bethesda, MD 20889-5600, USA. Tadakid@NMRC.Navy.mil
FAU - Williams, A
AU  - Williams A
FAU - Lee, K P
AU  - Lee KP
FAU - Kirk, A D
AU  - Kirk AD
FAU - Harlan, D M
AU  - Harlan DM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Denmark
TA  - Xenotransplantation
JT  - Xenotransplantation
JID - 9438793
RN  - 0 (B7-1 Antigen)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - B7-1 Antigen/chemistry/*genetics/*immunology
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Humans
MH  - Lymphocyte Activation/*immunology
MH  - Macaca mulatta
MH  - Models, Immunological
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Protein Biosynthesis
MH  - Recombinant Proteins/immunology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - T-Lymphocytes/*immunology
MH  - Transfection
MH  - Transplantation, Heterologous
EDAT- 2003/04/16 05:00
MHDA- 2004/04/09 05:00
CRDT- 2003/04/16 05:00
PHST- 2003/04/16 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2003/04/16 05:00 [entrez]
AID - 2o004 [pii]
AID - 10.1034/j.1399-3089.2003.02004.x [doi]
PST - ppublish
SO  - Xenotransplantation. 2003 May;10(3):252-8. doi: 10.1034/j.1399-3089.2003.02004.x.