PMID- 12694546
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20211203
IS  - 0908-665X (Print)
IS  - 0908-665X (Linking)
VI  - 10
IP  - 3
DP  - 2003 May
TI  - Construction of ectopic xenogeneic bone marrow structure associated with 
      persistent multi-lineage mixed chimerism by engraftment of rat bone marrow plugs 
      into mouse kidney capsules.
PG  - 259-66
AB  - Poor bone marrow (BM) engraftment in a xenogeneic combination results at least in 
      part from the limited engraftment capacity of BM-derived stromal cells, which 
      support hematopoietic repopulation in a species-specific fashion. We attempted to 
      construct a BM stromal microenvironment by engraftment of BM plug fragments into 
      kidney capsules in a rat-to-mouse combination. BM plugs from F344/N Jcl-rnu/rnu 
      (F344 nu) rats were transplanted into the kidney capsules of C.B-17 scid/scid 
      (C.B-17 scid) mice treated with rabbit anti-asialo-GM1 serum to deplete natural 
      killer (NK) cells and then with 3 Gy of whole body irradiation. As a conventional 
      control, an equivalent amount of F344 nu bone marrow cells (BMCs) was 
      intravenously injected into C.B-17 scid mice treated with a similar conditioning 
      regimen. In both mouse recipients of rat BM plug engraftment in the kidney 
      capsules and recipients of intravenous injection of rat BMC suspension, 
      comparable extents of donor rat class I+ cells were persistently detected in the 
      peripheral blood. However, the differentiation of rat-derived B cells in the 
      mouse recipients of rat BM plugs was more rapid than that in the recipients of 
      rat BMC suspension. In the late phase (10 weeks after BM transplantation), the 
      percentage of rat-derived T cells (CD4+ cells) in the mouse recipients of rat BM 
      plugs was significantly higher than that in the recipients of rat BMC suspension. 
      At this time point, ectopic BM structure consisting of bone, mesenchymal cells, 
      and hematopoietic progenitors was constructed in the kidney capsules of mice that 
      received rat BM plugs. Most of the cells in the ectopic BM were derived from the 
      donor rat. Thus, engraftment of BM plugs into the kidney capsules results in the 
      construction of a donor-derived BM microenvironment, facilitating multilineage 
      mixed xenogeneic chimerism.
FAU - Hara, Hidetaka
AU  - Hara H
AD  - Department of Surgery, Graduate School of Biomedical Sciences, Hiroshima 
      University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
FAU - Ohdan, Hideki
AU  - Ohdan H
FAU - Tokita, Daisuke
AU  - Tokita D
FAU - Onoe, Takashi
AU  - Onoe T
FAU - Zhou, Wendy
AU  - Zhou W
FAU - Asahara, Toshimasa
AU  - Asahara T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Xenotransplantation
JT  - Xenotransplantation
JID - 9438793
RN  - 0 (Antilymphocyte Serum)
SB  - IM
MH  - Animals
MH  - Antilymphocyte Serum/therapeutic use
MH  - Bone Marrow Cells/*cytology
MH  - Bone Marrow Transplantation/immunology/methods/*pathology
MH  - Immunosuppression Therapy
MH  - Killer Cells, Natural/immunology
MH  - Mice
MH  - Mice, SCID
MH  - Rats
MH  - Rats, Inbred F344
MH  - Subrenal Capsule Assay
MH  - *Transplantation Chimera
MH  - Transplantation, Heterologous/immunology/methods/*pathology
MH  - Whole-Body Irradiation
EDAT- 2003/04/16 05:00
MHDA- 2004/04/09 05:00
CRDT- 2003/04/16 05:00
PHST- 2003/04/16 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2003/04/16 05:00 [entrez]
AID - 2o026 [pii]
AID - 10.1034/j.1399-3089.2003.02026.x [doi]
PST - ppublish
SO  - Xenotransplantation. 2003 May;10(3):259-66. doi: 
      10.1034/j.1399-3089.2003.02026.x.