PMID- 12699815
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20191107
IS  - 1567-5394 (Print)
IS  - 1567-5394 (Linking)
VI  - 59
IP  - 1-2
DP  - 2003 Apr
TI  - Liposome-mediated conformation transition of DNA detected by molecular probe: 
      methyl green.
PG  - 21-7
AB  - Recent studies have focused on the structural features of DNA-lipid assemblies. 
      In this paper, we take methyl green (MG) as a probe molecule to detect the 
      conformational change of DNA molecule induced by dimethyldioctadecylammonium 
      bromide (DDAB) liposomes before the condensation process of DNA begins. 
      DDAB-induced DNA topology changes were investigated by cyclic voltammetry (CV), 
      circular dichroism (CD) and UV-VIS spectrometry. We find that upon binding to 
      DNA, positively charged liposomes induce a conformational transition of DNA 
      molecules from the native B-form to the C motif. Conformational transition in DNA 
      results in the binding modes of MG to DNA, changing and being isolated from DNA 
      to the solution. More stable complexes are formed between DNA and DDAB. That is 
      also proved by the melting study of DNA.
FAU - Huang, Weimin
AU  - Huang W
AD  - State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of 
      Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
FAU - Zhang, Zheling
AU  - Zhang Z
FAU - Han, Xiaojun
AU  - Han X
FAU - Tang, Jilin
AU  - Tang J
FAU - Wang, Jianguo
AU  - Wang J
FAU - Dong, Shaojun
AU  - Dong S
FAU - Wang, Erkang
AU  - Wang E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Bioelectrochemistry
JT  - Bioelectrochemistry (Amsterdam, Netherlands)
JID - 100953583
RN  - 0 (Coloring Agents)
RN  - 0 (Liposomes)
RN  - 0 (Molecular Probes)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 13146-86-6 (didodecyldimethylammonium)
RN  - 82-94-0 (Methyl Green)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Circular Dichroism
MH  - Coloring Agents/*chemistry
MH  - DNA/analysis/*chemistry
MH  - Electrochemistry
MH  - Liposomes
MH  - Methyl Green/*chemistry
MH  - *Molecular Probes
MH  - *Nucleic Acid Conformation
MH  - Quaternary Ammonium Compounds/chemistry
MH  - Spectrophotometry, Ultraviolet
MH  - Thymus Gland/metabolism
EDAT- 2003/04/18 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/04/18 05:00
PHST- 2003/04/18 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/04/18 05:00 [entrez]
AID - S1567539402001858 [pii]
AID - 10.1016/s1567-5394(02)00185-8 [doi]
PST - ppublish
SO  - Bioelectrochemistry. 2003 Apr;59(1-2):21-7. doi: 10.1016/s1567-5394(02)00185-8.