PMID- 12701917
OWN - NLM
STAT- MEDLINE
DCOM- 20030708
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 47
IP  - 5
DP  - 2003
TI  - Comparison of detection specificity of nitrifying bacteria in biofilm using 
      fluorescence in situ hybridization and in situ fluorescent antibody methods.
PG  - 129-32
AB  - The in situ fluorescent antibody and fluorescence in situ hybridization (FISH) 
      methods are very useful in the in situ detection of specific bacteria like 
      nitrifiers in a biofilm. In this study, simultaneous staining using the FISH and 
      in situ fluorescent antibody methods was examined. As a result, no specific 
      fluorescence was observed with either method when FISH was performed followed by 
      the in situ fluorescent antibody method; however, when the in situ fluorescent 
      antibody method was performed first followed by FISH, specific fluorescence was 
      observed in both cases. Moreover, it was suggested that the detection 
      specificities of FISH and the in situ fluorescent antibody method are almost 
      identical.
FAU - Noda, N
AU  - Noda N
AD  - National Institute of Advanced Industrial Science and Technology, Central 6, 
      1-1-1 Higashi, Tsukuba, Ibaraki 305-8666, Japan.
FAU - Ebie, Y
AU  - Ebie Y
FAU - Matsumura, M
AU  - Matsumura M
FAU - Tsuneda, S
AU  - Tsuneda S
FAU - Hirata, A
AU  - Hirata A
FAU - Inamori, Y
AU  - Inamori Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Antibodies, Monoclonal)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - *Biofilms
MH  - Environmental Monitoring/*methods
MH  - Eutrophication
MH  - Fluorescent Antibody Technique
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrogen/*metabolism
EDAT- 2003/04/19 05:00
MHDA- 2003/07/09 05:00
CRDT- 2003/04/19 05:00
PHST- 2003/04/19 05:00 [pubmed]
PHST- 2003/07/09 05:00 [medline]
PHST- 2003/04/19 05:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2003;47(5):129-32.