PMID- 12702468
OWN - NLM
STAT- MEDLINE
DCOM- 20030618
LR  - 20111117
IS  - 0003-3898 (Print)
IS  - 0003-3898 (Linking)
VI  - 61
IP  - 2
DP  - 2003 Mar-Apr
TI  - [Spectral karyotyping (SKY) principle, avantages and limitations].
PG  - 139-46
AB  - Banding karyotype is a routine technique, which allows the identification of 
      numerous aneusomy and/or aneuploidy in congenital diseases and cancers. However, 
      this analysis fails to detect small or complex chromosome rearrangements. 
      Molecular cytogenetic techniques like fluorescence in situ hybridization (FISH) 
      analysis can overlap these limitations. Particularly, multicolor karyotyping by 
      spectral karyotyping (SKY) may rectify or precise the conventional karyotype 
      results. With two examples, we present here, the principle, the indications and 
      the limits of this technique for constitutional and cancer chromosomal 
      abnormalities characterization. Moreover, we present an easy way to build 
      efficient sky probes with a best sensitivity than the probes classically used.
FAU - Belaud-Rotureau, M A
AU  - Belaud-Rotureau MA
AD  - Service d'histologie, embryologie et cytogenetique, Hopital Necker enfants 
      malades, Paris.
FAU - Elghezal, H
AU  - Elghezal H
FAU - Bernardin, C
AU  - Bernardin C
FAU - Sanlaville, D
AU  - Sanlaville D
FAU - Radford-Weiss, I
AU  - Radford-Weiss I
FAU - Raoul, O
AU  - Raoul O
FAU - Vekemans, M
AU  - Vekemans M
FAU - Romana, S P
AU  - Romana SP
LA  - fre
PT  - Case Reports
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Review
TT  - Le caryotype spectral (SKY): principe, avantages et limites en cytogenetique 
      constitutionnelle et tumorale.
PL  - France
TA  - Ann Biol Clin (Paris)
JT  - Annales de biologie clinique
JID - 2984690R
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Base Sequence
MH  - Child
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - DNA/genetics
MH  - *Genetic Markers
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Intellectual Disability/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Research
MH  - Sensitivity and Specificity
MH  - *Spectral Karyotyping
MH  - Trisomy
RF  - 18
EDAT- 2003/04/19 05:00
MHDA- 2003/06/19 05:00
CRDT- 2003/04/19 05:00
PHST- 2003/04/19 05:00 [pubmed]
PHST- 2003/06/19 05:00 [medline]
PHST- 2003/04/19 05:00 [entrez]
PST - ppublish
SO  - Ann Biol Clin (Paris). 2003 Mar-Apr;61(2):139-46.