PMID- 12702510
OWN - NLM
STAT- MEDLINE
DCOM- 20030911
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 102
IP  - 4
DP  - 2003 Aug 15
TI  - IgE alone stimulates mast cell adhesion to fibronectin via pathways similar to 
      those used by IgE + antigen but distinct from those used by Steel factor.
PG  - 1405-13
AB  - We recently demonstrated that immunoglobulin E (IgE), in the absence of 
      cross-linking agents, activates signaling pathways in healthy murine bone 
      marrow-derived mast cells (BMMCs) and that this activation enhances BMMC 
      survival, at least in part, via secretion of autocrine-acting cytokines. We 
      report herein that IgE alone also triggers the adhesion of both BMMCs and 
      connective tissue mast cells (CTMCs) to the connective tissue component, 
      fibronectin (FN). This adhesion occurs to the same extent as that triggered by 
      optimal levels of Steel factor (SF) or IgE + antigen (IgE + Ag) and is mediated 
      by an increased avidity of the integrin very late antigen 5 (VLA-5). Moreover, 
      this IgE-induced adhesion, which is prolonged compared with that elicited by SF 
      or IgE + Ag, requires phosphatidylinositol 3-kinase (PI3K), phospholipase C gamma 
      (PLCgamma), and extracellular calcium but not extracellular-regulated kinase 
      (Erk) or p38. Interestingly, we found, using the calcium channel blocker, 2-APB 
      (2-aminoethoxydiphenyl borate) and Lyn-/- BMMCs that both IgE- and IgE + 
      Ag-induced adhesion to FN require extracellular calcium entry, whereas SF does 
      not. Furthermore, our data suggest that FN acts synergistically with IgE to 
      prolong intracellular phosphorylation events and to enhance IgE-induced 
      inflammatory cytokine production and BMMC survival.
FAU - Lam, Vivian
AU  - Lam V
AD  - Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC, Canada.
FAU - Kalesnikoff, Janet
AU  - Kalesnikoff J
FAU - Lee, Corinna W K
AU  - Lee CW
FAU - Hernandez-Hansen, Valerie
AU  - Hernandez-Hansen V
FAU - Wilson, Bridget S
AU  - Wilson BS
FAU - Oliver, Janet M
AU  - Oliver JM
FAU - Krystal, Gerald
AU  - Krystal G
LA  - eng
GR  - GM-49814/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030417
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Boron Compounds)
RN  - 0 (Cytokines)
RN  - 0 (Fibronectins)
RN  - 0 (Integrin alpha5beta1)
RN  - 0 (Stem Cell Factor)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - E4ES684O93 (2-aminoethoxydiphenyl borate)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Bone Marrow Cells/cytology/immunology/metabolism
MH  - Boron Compounds/pharmacology
MH  - Calcium/metabolism
MH  - Cell Adhesion/physiology
MH  - Cytokines/biosynthesis
MH  - Fibronectins/immunology/*metabolism
MH  - Immunoglobulin E/immunology/*metabolism/*pharmacology
MH  - Integrin alpha5beta1/immunology/metabolism
MH  - Mast Cells/cytology/enzymology/immunology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phospholipase C gamma
MH  - Phosphorylation
MH  - Signal Transduction
MH  - Stem Cell Factor/immunology/*metabolism
MH  - Type C Phospholipases/metabolism
MH  - src-Family Kinases/deficiency/metabolism
EDAT- 2003/04/19 05:00
MHDA- 2003/09/13 05:00
CRDT- 2003/04/19 05:00
PHST- 2003/04/19 05:00 [pubmed]
PHST- 2003/09/13 05:00 [medline]
PHST- 2003/04/19 05:00 [entrez]
AID - S0006-4971(20)44311-3 [pii]
AID - 10.1182/blood-2002-10-3176 [doi]
PST - ppublish
SO  - Blood. 2003 Aug 15;102(4):1405-13. doi: 10.1182/blood-2002-10-3176. Epub 2003 Apr 
      17.