PMID- 12704659
OWN - NLM
STAT- MEDLINE
DCOM- 20030527
LR  - 20141120
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 195
IP  - 3
DP  - 2003 Jun
TI  - Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ 
      culture: possible involvement of endocytosis in mechanical stress-stimulated 
      osteoblast differentiation.
PG  - 488-96
AB  - We previously demonstrated that tensile stress (TS)-induced osteoblast 
      differentiation eventually led to osteogenesis in an organ culture of mouse 
      calvarial sutures. In the present study, we employed RNA-fingerprinting using an 
      arbitrarily primed polymerase chain reaction (RAP-PCR) to identify alpha-adaptin 
      C, a component of the endocytosis machinery AP2, as a TS-inducible gene. Protein 
      production, as well as the gene expression of alpha-adaptin C, was induced by TS 
      as early as 3 h following the initiation of loading. In situ hybridization and 
      immunohistochemical analysis revealed that the induction of alpha-adaptin C 
      mostly occurred in fibroblastic cells in the sutures, suggesting that it precedes 
      TS-induced osteoblast differentiation. Consistent with this result, TS 
      significantly increased the number of coated pits (CPs) and coated vesicles (CVs) 
      in the undifferentiated fibroblastic cells but not in the osteoblastic cells 
      around calvarial bones. Further, TS-induced osteoblast differentiation was 
      suppressed when endocytosis was inhibited by potassium depletion. These results, 
      taken together, suggest that TS accelerates osteoblast differentiation and 
      osteogenesis, possibly through the induction of the alpha-adaptin C expression 
      and consequent activation of receptor-mediated endocytosis.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Shimomura, Junko
AU  - Shimomura J
AD  - Division of Cell Biology and Molecular Pharmacology, Niigata University Graduate 
      School of Medical and Dental Sciences, Gakkocho-dori, Japan.
FAU - Ishibashi, Osamu
AU  - Ishibashi O
FAU - Ikegame, Mika
AU  - Ikegame M
FAU - Yoshizawa, Tatsuya
AU  - Yoshizawa T
FAU - Ejiri, Sadakazu
AU  - Ejiri S
FAU - Noda, Tadashi
AU  - Noda T
FAU - Kawashima, Hiroyuki
AU  - Kawashima H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Adaptor Protein Complex 2)
RN  - 0 (Adaptor Protein Complex alpha Subunits)
RN  - 0 (RNA, Messenger)
RN  - 0 (adaptor protein complex 2, alpha 2 subunit)
SB  - IM
MH  - Adaptor Protein Complex 2/analysis/*biosynthesis/genetics
MH  - Adaptor Protein Complex alpha Subunits/analysis/*biosynthesis/genetics
MH  - Animals
MH  - Cell Differentiation
MH  - Coated Pits, Cell-Membrane/ultrastructure
MH  - Coated Vesicles/ultrastructure
MH  - Cranial Sutures/anatomy & histology/metabolism/ultrastructure
MH  - Endocytosis
MH  - Gene Expression Regulation
MH  - Mice
MH  - Organ Culture Techniques
MH  - Osteoblasts/*cytology
MH  - *Osteogenesis
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Skull/cytology
MH  - Stress, Mechanical
EDAT- 2003/04/22 05:00
MHDA- 2003/05/28 05:00
CRDT- 2003/04/22 05:00
PHST- 2003/04/22 05:00 [pubmed]
PHST- 2003/05/28 05:00 [medline]
PHST- 2003/04/22 05:00 [entrez]
AID - 10.1002/jcp.10269 [doi]
PST - ppublish
SO  - J Cell Physiol. 2003 Jun;195(3):488-96. doi: 10.1002/jcp.10269.