PMID- 12706083
OWN - NLM
STAT- MEDLINE
DCOM- 20030529
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 308
IP  - 2
DP  - 2003 Apr 10
TI  - Characterization of the immediate-early 2 protein of human herpesvirus 6, a 
      promiscuous transcriptional activator.
PG  - 340-53
AB  - In the present work we report the cloning of a full-length cDNA encoding the 
      immediate-early (IE) 2 protein from human herpesvirus 6 (HHV-6) variant A (GS 
      strain). The transcript is 4690 nucleotides long and composed of 5 exons. 
      Translation initiation occurs within the third exon and proceeds to the end of 
      U86. Kinetic studies indicate that the 5.5-kb IE2 mRNA is expressed under IE 
      condition, within 2-4 h of infection. IE2 transcripts from both variants A and B 
      are expressed under similar kinetics with IE2 transcripts accumulating up to 96 h 
      postinfection. Although several large transcripts (>5.5 kb) hybridized with the 
      IE2 probe, suggesting multiple transcription initiation sites, a single form of 
      the IE2 protein, in excess of 200 kDa, was detected by Western blot. Within 
      cells, the IE2 protein was detected (8-48 h) as intranuclear granules while at 
      later time points (72-120 h), the IE2 protein coalesced into a few large 
      immunoreactive patches. Transfection of cells with an IE2 expression vector 
      (pBK-IE2A) failed to reproduce the patch-like distribution, suggesting that other 
      viral proteins are necessary for this process to occur. Last, IE2 was found to 
      behave as a promiscuous transcriptional activator. Cotransfection experiments in 
      T cells indicate that IE2 can induce the transcription of a complex promoter, 
      such as the HIV-LTR, as well as simpler promoters, whose expression is driven by 
      a unique set of responsive elements (CRE, NFAT, NF-kB). Moreover, minimal 
      promoters having a single TATA box or no defined eukaryotic regulatory elements 
      were significantly activated by IE2, suggesting that IE2 is likely to play an 
      important role in initiating the expression of several HHV-6 genes. In all, the 
      work presented represents the first report on the successful cloning, expression, 
      and functional characterization of the major regulatory IE2 gene/protein of 
      HHV-6.
FAU - Gravel, Annie
AU  - Gravel A
AD  - Laboratory of Virology, Rheumatology and Immunology Research Center, CHUL 
      Research Center and Faculty of Medicine, Laval University, Quebec, Canada.
FAU - Tomoiu, Andru
AU  - Tomoiu A
FAU - Cloutier, Nathalie
AU  - Cloutier N
FAU - Gosselin, Jean
AU  - Gosselin J
FAU - Flamand, Louis
AU  - Flamand L
LA  - eng
SI  - GENBANK/AY037931
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - HIV Long Terminal Repeat
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Immediate-Early Proteins/analysis/*genetics/physiology
MH  - Kinetics
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - TATA Box
MH  - Trans-Activators/*genetics
EDAT- 2003/04/23 05:00
MHDA- 2003/05/30 05:00
CRDT- 2003/04/23 05:00
PHST- 2003/04/23 05:00 [pubmed]
PHST- 2003/05/30 05:00 [medline]
PHST- 2003/04/23 05:00 [entrez]
AID - S0042682203000072 [pii]
AID - 10.1016/s0042-6822(03)00007-2 [doi]
PST - ppublish
SO  - Virology. 2003 Apr 10;308(2):340-53. doi: 10.1016/s0042-6822(03)00007-2.