PMID- 12707231
OWN - NLM
STAT- MEDLINE
DCOM- 20030505
LR  - 20231213
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 107
IP  - 16
DP  - 2003 Apr 29
TI  - Isolation of bone marrow stromal cell-derived smooth muscle cells by a human 
      SM22alpha promoter: in vitro differentiation of putative smooth muscle progenitor 
      cells of bone marrow.
PG  - 2078-81
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs) have many characteristics of 
      mesenchymal stem cells that can differentiate into smooth muscle cells (SMCs). 
      However, there have been few studies closely following the cell development of 
      smooth muscle lineage among BMSCs. METHODS AND RESULTS: To investigate the 
      possible existence of a cell population committed to the SMC lineage among bone 
      marrow adhesion cells, we tried to detect and follow the in vitro differentiation 
      of such a cell type by using a promoter-sorting method with a human SM22alpha 
      promoter (-480 bp)/green fluorescent protein (GFP) construct. The construct was 
      transfected to adhesion cells that appeared 5 days after the seeding of 
      mononuclear cells from bone marrow. GFP was first detectable 5 days after the 
      transfection in a cell population [Ad(G) cells], which expressed PDGF-beta but 
      neither mature (calponin) nor immature (SMemb) SMC-specific proteins at that 
      time. However, the cells were eventually grown into individual clones that 
      expressed SMC-specific proteins (alpha-smooth muscle actin, calponin, and SM-1), 
      suggesting that Ad(G) cells have partly at least progenitor properties. Because 
      early studies have reported that PDGF-beta signaling plays pivotal roles in the 
      differentiation of mesenchymal smooth muscle progenitor cells, Ad(G) cells might 
      be putative mesenchymal smooth muscle progenitors expressing PDGF-beta. 
      CONCLUSIONS: We demonstrated the presence of a cell population fated to become 
      SMCs and followed their differentiation into SMCs among BMSCs.
FAU - Kashiwakura, Yuji
AU  - Kashiwakura Y
AD  - Department of Cardiology, Juntendo University School of Medicine, 2-1-1 Hongo, 
      Bunkyo-ku, Tokyo 113-8421, Japan.
FAU - Katoh, Youichi
AU  - Katoh Y
FAU - Tamayose, Kenji
AU  - Tamayose K
FAU - Konishi, Hakuoh
AU  - Konishi H
FAU - Takaya, Norihide
AU  - Takaya N
FAU - Yuhara, Senji
AU  - Yuhara S
FAU - Yamada, Masanori
AU  - Yamada M
FAU - Sugimoto, Koichi
AU  - Sugimoto K
FAU - Daida, Hiroyuki
AU  - Daida H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030421
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Antibodies)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tagln protein, mouse)
RN  - 0 (transgelin)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
RN  - EC 3.6.1.- (Smooth Muscle Myosins)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Bone Marrow Cells/*cytology
MH  - Calcium-Binding Proteins/analysis/immunology
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Clone Cells
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/analysis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microfilament Proteins/*genetics
MH  - Muscle Proteins/*genetics
MH  - Muscle, Smooth, Vascular/chemistry/*cytology/metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, Platelet-Derived Growth Factor beta/analysis/immunology
MH  - Recombinant Fusion Proteins/analysis
MH  - Smooth Muscle Myosins/analysis/biosynthesis/genetics
MH  - Stem Cells/*physiology
MH  - Stromal Cells/*physiology
MH  - Transfection
MH  - Calponins
EDAT- 2003/04/23 05:00
MHDA- 2003/05/06 05:00
CRDT- 2003/04/23 05:00
PHST- 2003/04/23 05:00 [pubmed]
PHST- 2003/05/06 05:00 [medline]
PHST- 2003/04/23 05:00 [entrez]
AID - 01.CIR.0000070082.64414.B5 [pii]
AID - 10.1161/01.CIR.0000070082.64414.B5 [doi]
PST - ppublish
SO  - Circulation. 2003 Apr 29;107(16):2078-81. doi: 
      10.1161/01.CIR.0000070082.64414.B5. Epub 2003 Apr 21.