PMID- 12707375
OWN - NLM
STAT- MEDLINE
DCOM- 20031212
LR  - 20191107
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 5
IP  - 2
DP  - 2003 May
TI  - Chromosomal abnormalities in non-small cell lung carcinomas and in bronchial 
      epithelia of high-risk smokers detected by multi-target interphase fluorescence 
      in situ hybridization.
PG  - 103-12
AB  - Human lung carcinogenesis is accompanied by complex chromosomal changes that may 
      be detected in interphase cells by fluorescence in situ hybridization (FISH) 
      assay using recently developed multitarget DNA probes. Touch preparations of 20 
      non-small cell lung carcinomas, sputum specimens from 3 patients with lung cancer 
      and from 11 ex-smokers without lung cancer, and cultured benign bronchial 
      epithelium of 42 high-risk smokers, 9 of whom had concurrent invasive carcinoma, 
      were tested using a four-color FISH probe (LAVysion) targeting centromere 6, 
      5p15.2, 7p12 (EGFR), and 8q24 (MYC). Significantly high frequencies of abnormal 
      cells were found in each of the 20 NSCLC (100%) and in the 3 sputum specimens 
      from lung cancer patients. None of the cytologically normal sputa contained FISH 
      abnormalities. Cultured bronchial epithelial cells from 11 of 42 patients (26%) 
      were abnormal for at least one probe. Abnormal FISH patterns had no association 
      with gender, presence of tumor or histology. Multicolor FISH can readily detect 
      chromosomal abnormalities in imprints and sputa from lung carcinomas. Chromosomal 
      aneusomy is also frequent in bronchial epithelial cells from long-term smokers. 
      The prognostic significance of the multicolor LAVysion FISH probe set should be 
      validated in a controlled clinical trial.
FAU - Romeo, Maura Santos
AU  - Romeo MS
AD  - Department of Medicine, School of Medicine, University of Colorado Health 
      Sciences Center, Denver, Colorado 80262, USA.
FAU - Sokolova, Irina A
AU  - Sokolova IA
FAU - Morrison, Larry E
AU  - Morrison LE
FAU - Zeng, Chan
AU  - Zeng C
FAU - Baron, Anna E
AU  - Baron AE
FAU - Hirsch, Fred R
AU  - Hirsch FR
FAU - Miller, York E
AU  - Miller YE
FAU - Franklin, Wilbur A
AU  - Franklin WA
FAU - Varella-Garcia, Marileila
AU  - Varella-Garcia M
LA  - eng
GR  - U01 CA085070/CA/NCI NIH HHS/United States
GR  - P50 CA058187/CA/NCI NIH HHS/United States
GR  - P30 CA046934/CA/NCI NIH HHS/United States
GR  - P01-CA58187/CA/NCI NIH HHS/United States
GR  - U01-CA85070/CA/NCI NIH HHS/United States
GR  - P30-CA46934/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Bronchi/*metabolism
MH  - Carcinoma, Non-Small-Cell Lung/*genetics
MH  - *Chromosome Aberrations
MH  - DNA/metabolism
MH  - Epithelial Cells/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/*genetics
MH  - Microscopy, Fluorescence
MH  - Risk
MH  - *Smoking
MH  - Sputum/metabolism
PMC - PMC1907319
EDAT- 2003/04/23 05:00
MHDA- 2003/12/13 05:00
PMCR- 2004/05/01
CRDT- 2003/04/23 05:00
PHST- 2003/04/23 05:00 [pubmed]
PHST- 2003/12/13 05:00 [medline]
PHST- 2003/04/23 05:00 [entrez]
PHST- 2004/05/01 00:00 [pmc-release]
AID - S1525-1578(10)60459-X [pii]
AID - 0123 [pii]
AID - 10.1016/s1525-1578(10)60459-x [doi]
PST - ppublish
SO  - J Mol Diagn. 2003 May;5(2):103-12. doi: 10.1016/s1525-1578(10)60459-x.