PMID- 12707777
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20181113
IS  - 0937-9827 (Print)
IS  - 0937-9827 (Linking)
VI  - 117
IP  - 3
DP  - 2003 Jun
TI  - Real-time PCR quantitation of FE65 a beta-amyloid precursor protein-binding 
      protein after traumatic brain injury in rats.
PG  - 153-9
AB  - In cases of traumatic brain injury (TBI) in which the patient survived for only a 
      short period of time and was without macroscopic changes at autopsy, it is 
      difficult to diagnose TBI. To detect early diagnostic markers of diffuse axonal 
      injury (DAI), real-time quantitative reverse transcriptase-polymerase chain 
      reaction (RT-PCR) in an experimental head trauma model of rat was chosen. The 
      beta-amyloid precursor protein (beta-APP) is a well-known diagnostic marker of 
      DAI which can be detected by immunolabeling as early as 1.5 h after injury. 
      beta-APP has a binding protein, FE65, which is expressed in the brain of 
      Alzheimer's disease patients along with beta-APP, but no involvement with brain 
      injury has been reported. Neuron-specific enolase (NSE) is also a useful marker 
      of DAI. We found that FE65 expression increased dramatically as early as 30 min 
      after injury and decreased after peaking 1 h post-injury, although NSE showed no 
      significant changes. These results suggest that real-time PCR of FE65 mRNA is 
      useful for the diagnosis of DAI in forensic cases.
FAU - Iino, Morio
AU  - Iino M
AD  - Department of Legal Medicine, Osaka University Graduate School of Medicine, 
      2-2-F3 Yamada-oka, Suita, 565-0871 Osaka, Japan. iino@legal.med.osaka-u.ac.jp
FAU - Nakatome, Masato
AU  - Nakatome M
FAU - Ogura, Yoshiaki
AU  - Ogura Y
FAU - Fujimura, Harutoshi
AU  - Fujimura H
FAU - Kuroki, Hisanaga
AU  - Kuroki H
FAU - Inoue, Hiromasa
AU  - Inoue H
FAU - Ino, Yukiko
AU  - Ino Y
FAU - Fujii, Tasuku
AU  - Fujii T
FAU - Terao, Toshiyuki
AU  - Terao T
FAU - Matoba, Ryoji
AU  - Matoba R
LA  - eng
PT  - Journal Article
DEP - 20030418
PL  - Germany
TA  - Int J Legal Med
JT  - International journal of legal medicine
JID - 9101456
RN  - 0 (APBB1 protein, human)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Apbb1 protein, rat)
RN  - 0 (Biomarkers)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Animals
MH  - Autopsy
MH  - Biomarkers/analysis
MH  - Diffuse Axonal Injury/*metabolism/pathology
MH  - Humans
MH  - Male
MH  - Nerve Tissue Proteins/*analysis/biosynthesis
MH  - Nuclear Proteins/*analysis/biosynthesis
MH  - Protein Binding
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
EDAT- 2003/04/23 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/04/23 05:00
PHST- 2002/09/22 00:00 [received]
PHST- 2003/03/04 00:00 [accepted]
PHST- 2003/04/23 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/04/23 05:00 [entrez]
AID - 10.1007/s00414-003-0370-y [doi]
PST - ppublish
SO  - Int J Legal Med. 2003 Jun;117(3):153-9. doi: 10.1007/s00414-003-0370-y. Epub 2003 
      Apr 18.