PMID- 12708746
OWN - NLM
STAT- MEDLINE
DCOM- 20040114
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 245
IP  - 1-2
DP  - 2003 Mar
TI  - Norepinephrine-induced expression of cytokines in isolated biventricular working 
      rat hearts.
PG  - 69-76
AB  - The norepinephrine (NE)-induced hypertrophy of the left ventricle (LV) in the rat 
      is associated with increased interleukin (IL)-6 and IL-1beta expression. In the 
      present study, a newly established model of isolated biventricular working rat 
      heart was used to examine whether NE may directly induce cytokine mRNA expression 
      in a preparation devoid of other circulating hormonal and humoral factors. 
      Representative hemodynamic parameters and the expression of various cytokines of 
      the isolated biventricular working heart (IBWH) were compared with the respective 
      in vivo results. Systolic pressure (SP) of the right ventricle (RVSP) was higher 
      in the IBWH than in the intact anesthetized rat (42.9 +/- 1.89 vs. 32.3 +/- 
      1.06). However, heart rate (HR), LVSP and the maximal rate of pressure 
      development of LV (LV dP/dt(max)) were lower. After NE infusion (30 nM), SP and 
      dP/dt(max) were increased by 30 and 90%, respectively, in both ventricles. In 
      vivo, the ventricles showed a different response to NE (0.1 mg/kg x h): LVSP 
      increased by 15%, RVSP and RV dP/dt(max) was doubled, LV dP/dt(max) was tripled. 
      The analysis of cytokine mRNA expression with the RNase protection assay revealed 
      that in vivo IL-6 and IL-1beta were increased between 4 and 12 h 80- and 12-fold, 
      respectively, while there was weak expression under control conditions. In the 
      IBWH IL- 1alpha, IL-1beta, IL-6 and tumor necrosis factor (TNF)alpha were 
      increased already during control perfusion. The increase of these 
      stress-activated cytokines indicates that the isolation and perfusion procedure 
      may exert a stress on the heart. NE induced an additional time-dependent increase 
      of IL-6 mRNA after 1 h of infusion. Thus, NE has a direct effect on the cardiac 
      IL-6 expression, which occurred earlier in the in vitro preparation than in the 
      rat heart in vivo.
FAU - Briest, Wilfried
AU  - Briest W
AD  - Carl-Ludwig-Institute of Physiology, University of Leipzig, Leipzig, Germany. 
      briestw@medizin.uni-leipzig.de
FAU - Elsner, Christian
AU  - Elsner C
FAU - Hemker, Jan
AU  - Hemker J
FAU - Muller-Strahl, Gerhard
AU  - Muller-Strahl G
FAU - Zimmer, Heinz-Gerd
AU  - Zimmer HG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Cytokines/drug effects/genetics/*metabolism
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Heart/*drug effects
MH  - Heart Rate/drug effects
MH  - Heart Ventricles/drug effects
MH  - Interleukin-1/genetics/metabolism
MH  - Interleukin-6/genetics/metabolism
MH  - Myocardial Contraction/*drug effects/*physiology
MH  - Norepinephrine/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
EDAT- 2003/04/24 05:00
MHDA- 2004/01/15 05:00
CRDT- 2003/04/24 05:00
PHST- 2003/04/24 05:00 [pubmed]
PHST- 2004/01/15 05:00 [medline]
PHST- 2003/04/24 05:00 [entrez]
AID - 10.1023/a:1022861609896 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2003 Mar;245(1-2):69-76. doi: 10.1023/a:1022861609896.