PMID- 12708978
OWN - NLM
STAT- MEDLINE
DCOM- 20030902
LR  - 20190823
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 58
IP  - 4
DP  - 2003 Apr
TI  - IgE-reactivity to seven Malassezia species.
PG  - 306-11
AB  - BACKGROUND: Malassezia yeasts play a role in the pathogenesis of atopic 
      eczema/dermatitis syndrome (AEDS). The revised genus Malassezia includes several 
      species which all are natural habitants of the human skin. In this study, we 
      evaluated the presence of immunoglobulin E (IgE) antibodies to different 
      Malassezia spp. in AEDS patients to allow optimization of the characterization of 
      the IgE antibody profile of IgE-associated AEDS. METHODS: Ninety-six adult 
      patients, with a clinical diagnosis of AEDS, were included in the study. 
      Seventeen of the patients had IgE antibodies to M. sympodialis, ATCC 42132 (m70 
      ImmunoCAP, Pharmacia, Diagnostic AB, Uppsala, Sweden). The IgE antibodies to 
      seven Malassezia spp. were measured and inhibition immunoblotting was performed 
      to investigate whether M. sympodialis contains all the allergen components 
      present in the other Malassezia spp. RESULTS: Twenty per cent of 79 AEDS patients 
      with a negative m70 ImmunoCAP test had IgE antibodies to at least one of the 
      other six Malassezia spp. tested. Our inhibition studies indicated that 
      Malassezia spp. to a great extent, share allergenic determinants. However, 
      Malassezia species also contained species-specific allergens. CONCLUSION: The use 
      of only one species of Malassezia is not sufficient to detect all patients IgE 
      sensitized to Malassezia. To obtain an optimal allergen preparation both common 
      allergenic components as well as species-specific allergens have to be 
      considered.
FAU - Zargari, A
AU  - Zargari A
AD  - Ludwig Institute for Cancer Research, Stockholm Branch, Stockholm, Sweden.
FAU - Midgley, G
AU  - Midgley G
FAU - Back, O
AU  - Back O
FAU - Johansson, S G O
AU  - Johansson SG
FAU - Scheynius, A
AU  - Scheynius A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Antibodies, Fungal)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Fungal/*blood
MH  - Dermatitis, Atopic/*immunology/microbiology
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/*blood
MH  - Malassezia/*immunology/isolation & purification
MH  - Male
MH  - Middle Aged
EDAT- 2003/04/24 05:00
MHDA- 2003/09/03 05:00
CRDT- 2003/04/24 05:00
PHST- 2003/04/24 05:00 [pubmed]
PHST- 2003/09/03 05:00 [medline]
PHST- 2003/04/24 05:00 [entrez]
AID - 082 [pii]
AID - 10.1034/j.1398-9995.2003.00082.x [doi]
PST - ppublish
SO  - Allergy. 2003 Apr;58(4):306-11. doi: 10.1034/j.1398-9995.2003.00082.x.