PMID- 12710495
OWN - NLM
STAT- MEDLINE
DCOM- 20030515
LR  - 20200422
IS  - 0931-1793 (Print)
IS  - 1439-0450 (Electronic)
IS  - 0931-1793 (Linking)
VI  - 50
IP  - 1
DP  - 2003 Feb
TI  - Experimental dual infection of specific pathogen-free pigs with porcine 
      reproductive and respiratory syndrome virus and pseudorabies virus.
PG  - 14-9
AB  - Twenty 6-week-old specific pathogen-free pigs were divided into four groups. On 
      day 0 of the experiment, PRRSV-PRV (n = 6) and PRRSV (n = 4) groups were 
      intranasally inoculated with porcine reproductive and respiratory syndrome virus 
      (PRRSV) (10(5.6) TCID50). On day 7, the PRRSV-PRV and PRV (n = 6) groups were 
      intranasally inoculated with pseudorabies virus (PRV) (10(3.6) TCID50). Control 
      pigs (n = 4) were kept as uninoculated negative controls. Half of the pigs in 
      each group were euthanized and necropsied on day 14 or 21. Clinical signs such as 
      depression and anorexia were observed in the PRRSV-PRV and PRV groups after 
      inoculation with PRV. Although febrile response was observed after virus 
      inoculations, the duration of that response was prolonged in the PRRSV-PRV group 
      compared with the other groups. The lungs in the PRRSV-PRV group failed to 
      collapse and were mottled or diffusely tan and red, whereas the lungs of the pigs 
      in the other groups were grossly normal. Histopathologically, interstitial 
      pneumonia was present in all PRRSV-inoculated pigs, but the pneumonic lesions 
      were more severe in the PRRSV-PRV group. Mean PRRSV titres of tonsil and lung in 
      the PRRSV-PRV group were significantly (P < 0.05) higher than that in the PRRSV 
      group on day 21. These results indicate that dual infection with PRRSV and PRV 
      increased clinical signs and pneumonic lesions in pigs infected with both 
      viruses, as compared to pigs infected with PRRSV or PRV only, at least in the 
      present experimental conditions.
FAU - Shibata, I
AU  - Shibata I
AD  - Zen-noh Institute of Animal Health, Sakura, Chiba, Japan. 
      shibatai@zk.zennoh.or.jp
FAU - Yazawa, S
AU  - Yazawa S
FAU - Ono, M
AU  - Ono M
FAU - Okuda, Y
AU  - Okuda Y
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Vet Med B Infect Dis Vet Public Health
JT  - Journal of veterinary medicine. B, Infectious diseases and veterinary public 
      health
JID - 100955260
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Administration, Intranasal
MH  - Animals
MH  - Antibodies, Viral/*biosynthesis
MH  - Herpesvirus 1, Suid/*pathogenicity
MH  - Lung/pathology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Pseudorabies/*immunology/pathology
MH  - Specific Pathogen-Free Organisms
MH  - Swine/immunology
PMC - PMC7165977
EDAT- 2003/04/25 05:00
MHDA- 2003/05/16 05:00
PMCR- 2020/04/18
CRDT- 2003/04/25 05:00
PHST- 2003/04/25 05:00 [pubmed]
PHST- 2003/05/16 05:00 [medline]
PHST- 2003/04/25 05:00 [entrez]
PHST- 2020/04/18 00:00 [pmc-release]
AID - ZPH605 [pii]
AID - 10.1046/j.1439-0450.2003.00605.x [doi]
PST - ppublish
SO  - J Vet Med B Infect Dis Vet Public Health. 2003 Feb;50(1):14-9. doi: 
      10.1046/j.1439-0450.2003.00605.x.