PMID- 12710870
OWN - NLM
STAT- MEDLINE
DCOM- 20030529
LR  - 20190916
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 159
IP  - 5
DP  - 2003 May
TI  - Radiation-induced chromosome aberrations in ataxia telangiectasia cells: high 
      frequency of deletions and misrejoining detected by fluorescence in situ 
      hybridization.
PG  - 597-603
AB  - The mechanisms underlying the hyper-radiosensitivity of AT cells were 
      investigated by analyzing chromosome aberrations in the G(2) and M phases of the 
      cell cycle using a combination of chemically induced premature chromosome 
      condensation (PCC) and fluorescence in situ hybridization (FISH) with chromosome 
      painting probes. Confluent cultures of normal fibroblast cells (AG1522) and 
      fibroblast cells derived from an individual with AT (GM02052) were exposed to 
      gamma rays and allowed to repair at 37 degrees C for 24 h. At doses that resulted 
      in 10% survival, GM02052 cells were approximately five times more sensitive to 
      gamma rays than AG1522 cells. For a given dose, GM02052 cells contained a much 
      higher frequency of deletions and misrejoining than AG1522 cells. For both cell 
      types, a good correlation was found between the percentage of aberrant cells and 
      cell survival. The average number of color junctions, which represent the 
      frequency of chromosome misrejoining, was also found to correlate well with 
      survival. However, in a similar surviving population of GM02052 and AG1522 cells, 
      induced by 1 Gy and 6 Gy, respectively, AG1522 cells contained four times more 
      color junctions and half as many deletions as GM02052 cells. These results 
      indicate that both repair deficiency and misrepair may be involved in the 
      hyper-radiosensitivity of AT cells.
FAU - Kawata, Tetsuya
AU  - Kawata T
AD  - Department of Radiology, Graduate School of Medicine, Chiba University, Chiba, 
      Japan. tkawata@med.m.chiba-u.ac.jp
FAU - Ito, Hisao
AU  - Ito H
FAU - George, Kerry
AU  - George K
FAU - Wu, Honglu
AU  - Wu H
FAU - Uno, Takashi
AU  - Uno T
FAU - Isobe, Kouichi
AU  - Isobe K
FAU - Cucinotta, Francis A
AU  - Cucinotta FA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Ataxia Telangiectasia/*genetics
MH  - Cells, Cultured
MH  - *Chromosome Deletion
MH  - DNA Damage
MH  - *DNA Repair
MH  - G2 Phase
MH  - Gamma Rays
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Cucinotta, F A
IR  - Cucinotta FA
IRAD- NASA JSC
EDAT- 2003/04/25 05:00
MHDA- 2003/05/30 05:00
CRDT- 2003/04/25 05:00
PHST- 2003/04/25 05:00 [pubmed]
PHST- 2003/05/30 05:00 [medline]
PHST- 2003/04/25 05:00 [entrez]
AID - 10.1667/0033-7587(2003)159[0597:rcaiat]2.0.co;2 [doi]
PST - ppublish
SO  - Radiat Res. 2003 May;159(5):597-603. doi: 
      10.1667/0033-7587(2003)159[0597:rcaiat]2.0.co;2.