PMID- 12712095
OWN - NLM
STAT- MEDLINE
DCOM- 20030522
LR  - 20190616
IS  - 0002-9378 (Print)
IS  - 0002-9378 (Linking)
VI  - 188
IP  - 4
DP  - 2003 Apr
TI  - Expression of regulator of G protein signaling-2 in rat myometrium during 
      pregnancy and parturition.
PG  - 973-7
AB  - OBJECTIVE: The purpose of this study was to determine the potential physiologic 
      roles of myometrial regulator of G protein signaling-2 (RGS2), a G 
      protein-associated GTPase, by the analysis of the changes in RGS2 messenger RNA 
      expression during pregnancy and parturition and to examine factors that regulate 
      these changes. STUDY DESIGN: Myometrial RGS2 messenger RNA levels were analyzed 
      by Northern blotting in rats (1). during pregnancy, parturition, and in the 
      postpartum period; (2). with preterm-induced and delayed, postterm delivery; (3). 
      that were ovariectomized and treated with either estradiol, progesterone, or 
      both; and (4). with unilateral uterine pregnancies. RESULTS: RGS2 messenger RNA 
      was almost undetectable until day 5 of pregnancy, when it rose sharply and 
      remained elevated up to and including day 19, at the time that progesterone 
      withdrawal occurs. The expression of myometrial RGS2 messenger RNA on day 22 did 
      not differ between rats either before or during delivery. Onapristone caused 
      preterm delivery and a premature fall in RGS2 messenger RNA levels. In contrast, 
      progesterone treatment prolonged pregnancy beyond day 25 and attenuated the 
      decline in RGS2 messenger RNA levels. Simulation of the first 5 days of pregnancy 
      resulted in a 3-fold rise in RGS2 messenger RNA expression. The levels of RGS2 in 
      nonimplanted horns were approximately one half that of pregnant horns. 
      CONCLUSION: Sex steroids, in particular progesterone, and the presence of the 
      conceptus play a role in the regulation of myometrial RGS2 messenger RNA 
      expression. Although the elevated myometrial RGS2 messenger RNA expression 
      corresponds to the period during pregnancy when the uterus is relatively 
      quiescent and the down-regulation of RGS2 messenger RNA at the end of pregnancy 
      may be related to the timing of parturition, the specific role of myometrial RGS2 
      remains unknown.
FAU - Suarez, Victor R
AU  - Suarez VR
AD  - Division of Maternal Fetal Medicine, University of Texas Medical Branch, 
      Galveston, USA.
FAU - Park, Eun-Sung
AU  - Park ES
FAU - Hankins, Gary D V
AU  - Hankins GD
FAU - Soloff, Melvyn S
AU  - Soloff MS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Obstet Gynecol
JT  - American journal of obstetrics and gynecology
JID - 0370476
RN  - 0 (Gonanes)
RN  - 0 (Hormone Antagonists)
RN  - 0 (RGS Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rgs2 protein, mouse)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - H6H7G23O3N (onapristone)
SB  - IM
MH  - Animals
MH  - Down-Regulation
MH  - Embryo Implantation
MH  - Female
MH  - Gonanes/pharmacology
MH  - Hormone Antagonists/pharmacology
MH  - Myometrium/*metabolism
MH  - Obstetric Labor, Premature/chemically induced
MH  - Parturition/*metabolism
MH  - Pregnancy
MH  - Pregnancy, Animal/drug effects/*metabolism
MH  - Pregnancy, Prolonged
MH  - Progesterone/pharmacology
MH  - RGS Proteins/genetics/*metabolism
MH  - RNA, Messenger/antagonists & inhibitors/metabolism
MH  - Rats
EDAT- 2003/04/25 05:00
MHDA- 2003/05/23 05:00
CRDT- 2003/04/25 05:00
PHST- 2003/04/25 05:00 [pubmed]
PHST- 2003/05/23 05:00 [medline]
PHST- 2003/04/25 05:00 [entrez]
AID - S0002937802715603 [pii]
AID - 10.1067/mob.2003.240 [doi]
PST - ppublish
SO  - Am J Obstet Gynecol. 2003 Apr;188(4):973-7. doi: 10.1067/mob.2003.240.