PMID- 12712358
OWN - NLM
STAT- MEDLINE
DCOM- 20030724
LR  - 20181113
IS  - 0721-832X (Print)
IS  - 0721-832X (Linking)
VI  - 241
IP  - 5
DP  - 2003 May
TI  - Plasma and whole-blood chemokine levels in patients with Behcet's disease.
PG  - 353-8
AB  - BACKGROUND: Chemokines are a family of chemoattractants of leukocytes that play a 
      critical role for leukocyte recruitment in various inflammatory diseases. The 
      purpose of this study is to investigate the involvement of chemokines, 
      interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in the 
      peripheral blood, with a special reference to disease activities of the patients 
      with Behcet's disease (BD). METHODS: The study population consisted of totally 55 
      patients with BD who had panuveitis (20 patients with active BD, 35 patients with 
      inactive BD) as well as 19 healthy volunteers as control. Disease activity was 
      defined according to the existence of ocular inflammation. IL-8 and MCP-1 
      concentration levels in the plasma and whole-blood samples were measured by 
      enzyme-linked immunosorbent assay. Whole-blood samples were obtained by lysing 
      cell membranes of peripheral blood cells. RESULTS: Most of the plasma IL-8 
      samples were below the detectable limit. Whole-blood IL-8 levels were readily 
      measured. The levels in the patients with active BD were significantly higher 
      than the other two groups. The patients with active and inactive BD showed higher 
      plasma and whole-blood levels of MCP-1 than controls. The plasma and whole-blood 
      MCP-1 levels of the samples collected at the same time showed a linear 
      correlation. CONCLUSION: A close relationship was found to exist between the 
      cell-associated IL-8 and the disease activity, while a persistent role of MCP-1 
      was observed in BD. Measuring the whole-blood levels of chemokines is useful for 
      monitoring the disease activity.
FAU - Kaburaki, Toshikatsu
AU  - Kaburaki T
AD  - Department of Ophthalmology, University of Tokyo Graduate School of Medicine, 
      7-3-1 Hongo, Bunkyo-ku, 113-8655 Tokyo, Japan. kabutosi-tky@umin.ac.jp
FAU - Fujino, Yujiro
AU  - Fujino Y
FAU - Kawashima, Hidetoshi
AU  - Kawashima H
FAU - Merino, Guillermo
AU  - Merino G
FAU - Numaga, Jiro
AU  - Numaga J
FAU - Chen, Jun
AU  - Chen J
FAU - Matsushima, Kouji
AU  - Matsushima K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030423
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glucocorticoids)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Behcet Syndrome/*blood/drug therapy/physiopathology
MH  - Chemokine CCL2/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Glucocorticoids/therapeutic use
MH  - Humans
MH  - Immunosuppressive Agents/therapeutic use
MH  - Interleukin-8/*blood
MH  - Male
MH  - Middle Aged
EDAT- 2003/04/25 05:00
MHDA- 2003/07/25 05:00
CRDT- 2003/04/25 05:00
PHST- 2002/11/18 00:00 [received]
PHST- 2003/02/18 00:00 [revised]
PHST- 2003/03/12 00:00 [accepted]
PHST- 2003/04/25 05:00 [pubmed]
PHST- 2003/07/25 05:00 [medline]
PHST- 2003/04/25 05:00 [entrez]
AID - 10.1007/s00417-003-0668-y [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2003 May;241(5):353-8. doi: 
      10.1007/s00417-003-0668-y. Epub 2003 Apr 23.