PMID- 12713462
OWN - NLM
STAT- MEDLINE
DCOM- 20031209
LR  - 20191107
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 5
IP  - 5
DP  - 2003 May
TI  - Structure and activity of multiple nitrifying bacterial populations co-existing 
      in a biofilm.
PG  - 355-69
AB  - A biofilm from a nitrifying pilot-scale sequencing batch reactor was investigated 
      for effects of varying process conditions on its microscale activity and 
      structure. Microsensor measurements of oxygen, substrates and products of 
      nitrification were applied under incubation at different ammonium and oxygen 
      concentrations which reflected various situations during a treatment cycle. A 
      high net N loss was observed under high ammonium (HA) concentrations in contrast 
      to low ones. Additionally, results indicated inhibition of nitrite-oxidizing 
      bacteria (NOB), but not of ammonia-oxidizing bacteria (AOB) by free ammonia under 
      HA conditions. Diversity, spatial distribution, and abundance of nitrifying 
      bacteria as analysed by fluorescence in situ hybridization (FISH) revealed six 
      different nitrifying populations with heterogeneous distributions. Nitrosococcus 
      mobilis formed conspicuous microcolonies locally surrounded by cells of the 
      dominating N. europaea/eutropha-related AOB population. A third less abundant 
      population was affiliated to N. oligotropha. Nitrite-oxidizing bacteria of the 
      genera Nitrobacter and Nitrospira (with at least two distinct populations) showed 
      a large scale heterogeneity in their distribution. Nitrospira spp. were also 
      found in deeper inactive layers where they might persist rather than thrive, and 
      act as seed population when detached. Results of functional and structural 
      analyses are discussed with respect to specific niches of individual populations 
      in this system.
FAU - Gieseke, Armin
AU  - Gieseke A
AD  - Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, 
      Celsiusstrasse 1, D-28359 Bremen, Germany. agieseke@mpi-bremen.de
FAU - Bjerrum, Lotte
AU  - Bjerrum L
FAU - Wagner, Michael
AU  - Wagner M
FAU - Amann, Rudolf
AU  - Amann R
LA  - eng
SI  - GENBANK/Y14636
SI  - GENBANK/Y14643
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Sewage)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/metabolism
MH  - *Bacterial Physiological Phenomena
MH  - *Biofilms
MH  - Bioreactors
MH  - Chromatiaceae/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Nitrates/*metabolism
MH  - Nitrites/*metabolism
MH  - Nitrobacter/*physiology
MH  - Nitrosomonas/*physiology
MH  - Nitrosomonas europaea/physiology
MH  - Oxygen/metabolism
MH  - Pilot Projects
MH  - Sewage/chemistry
MH  - Water Microbiology
EDAT- 2003/04/26 05:00
MHDA- 2003/12/10 05:00
CRDT- 2003/04/26 05:00
PHST- 2003/04/26 05:00 [pubmed]
PHST- 2003/12/10 05:00 [medline]
PHST- 2003/04/26 05:00 [entrez]
AID - 423 [pii]
AID - 10.1046/j.1462-2920.2003.00423.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2003 May;5(5):355-69. doi: 10.1046/j.1462-2920.2003.00423.x.