PMID- 12717265
OWN - NLM
STAT- MEDLINE
DCOM- 20040329
LR  - 20190906
IS  - 1536-4828 (Electronic)
IS  - 0885-3177 (Linking)
VI  - 26
IP  - 4
DP  - 2003 May
TI  - Association of plasma levels of tumor necrosis factor (TNF)-alpha and its soluble 
      receptors, two polymorphisms of the TNF gene, with acute severe pancreatitis and 
      early septic shock due to it.
PG  - 339-43
AB  - INTRODUCTION: Tumor necrosis factor-alpha (TNF-alpha) has been implicated in 
      acute severe pancreatitis (ASP). NcoI polymorphism exists in TNF gene promoter 
      and influences TNF-alpha gene transcription. AIMTo determine the predictive value 
      of plasma levels of TNF-alpha and its soluble receptors (sTNFR), TNF-alpha-308 
      and TNFB polymorphisms on the occurrence of ASP and ASP-associated early septic 
      shock. METHODOLOGY: Genotypes were determined in patients (n = 208) and healthy 
      controls (n = 116) by means of restriction fragment length polymorphism analysis 
      of polymerase chain reaction products. Plasma concentrations of TNF-alpha and 
      sTNFR were measured by ELISA. RESULTS: No significant differences were found in 
      baseline concentrations of TNF-alpha and sTNFR between patients who had early 
      septic shock and those who did not. Baseline TNF-alpha levels did not differ 
      significantly in patients displaying different alleles of the TNF gene. The 
      overall TNF2 allele frequency and TNFB2 allele frequency in patients with ASP 
      were both comparable with those in controls. TNF2 frequency was significantly 
      higher in patients with septic shock than in patients without early septic shock 
      (53.8% versus 22.4%; p = 0.003), as was TNFB2 frequency (88.5% versus 63.2%; p = 
      0.015). CONCLUSION: The study found no association between ASP and the two 
      polymorphisms examined, although some associations were found between TNF2 and 
      TNFB2 alleles with the development of early septic shock in ASP. Plasma level of 
      TNF-alpha or sTNFR was of little value in predicting the occurrence of early 
      septic shock in ASP.
FAU - Dianliang, Zhang
AU  - Dianliang Z
AD  - Department of General Surgery, School of Medicine, Nanjing University, Nanjing, 
      People's Republic of China. phdzdl@yahoo.com
FAU - Jieshou, Li
AU  - Jieshou L
FAU - Zhiwei, Jiang
AU  - Zhiwei J
FAU - Baojun, Yu
AU  - Baojun Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TNF protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.- (endodeoxyribonuclease NcoI)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Aged
MH  - DNA/genetics/metabolism
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Female
MH  - Gene Frequency
MH  - Humans
MH  - Lymphotoxin-alpha/genetics
MH  - Male
MH  - Middle Aged
MH  - Pancreatitis/blood/genetics/*pathology
MH  - Polymorphism, Genetic
MH  - Receptors, Tumor Necrosis Factor/*blood
MH  - Shock, Septic/blood/genetics/*pathology
MH  - Tumor Necrosis Factor-alpha/*genetics/*metabolism
EDAT- 2003/04/30 05:00
MHDA- 2004/03/30 05:00
CRDT- 2003/04/30 05:00
PHST- 2003/04/30 05:00 [pubmed]
PHST- 2004/03/30 05:00 [medline]
PHST- 2003/04/30 05:00 [entrez]
AID - 10.1097/00006676-200305000-00005 [doi]
PST - ppublish
SO  - Pancreas. 2003 May;26(4):339-43. doi: 10.1097/00006676-200305000-00005.