PMID- 12717617
OWN - NLM
STAT- MEDLINE
DCOM- 20030604
LR  - 20061115
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 187
IP  - 9
DP  - 2003 May 1
TI  - Human herpesvirus 6 genome and antigen in acute multiple sclerosis lesions.
PG  - 1365-76
AB  - Evidence for a candidate multiple sclerosis (MS) virus, human herpesvirus 6 
      (HHV-6), was sought in biopsy specimens of acute lesions that presented 
      clinically as cerebral tumors obtained from 5 patients. Histopathology, magnetic 
      resonance imaging, and clinical course confirmed the diagnosis of MS in each 
      case. A sensitive in situ polymerase chain reaction (ISPCR) method was used to 
      detect HHV-6 genome, in conjunction with immunocytochemical staining (ICC) to 
      detect viral and cellular antigens. ISPCR revealed numerous oligodendrocytes, 
      lymphocytes, and microglia containing HHV-6 genome within all lesions, whereas 
      ICC showed only the HHV-6 glycoprotein 116 antigen in some reactive astrocytes 
      and microglia. High frequencies of neuroglial and inflammatory cells containing 
      HHV-6 genome were present in acute-phase lesion tissue from patients who were 
      free of the effects of chronic MS and had not been received immunomodulatory 
      therapy for MS. The prevalence of HHV-6 genome-containing cells, including 
      oligodendrocytes, in each lesion suggests that HHV-6 plays a role in the 
      demyelinative pathogenesis of MS; the significance of the discrepant expression 
      of viral antigens remains uncertain.
FAU - Goodman, Andrew D
AU  - Goodman AD
AD  - Department of Neurology, University of Rochester School of Medicine and 
      Dentistry, Rochester, New York 14642, USA. andrew_goodman@urmc.rochester.edu
FAU - Mock, David J
AU  - Mock DJ
FAU - Powers, James M
AU  - Powers JM
FAU - Baker, Jeffrey V
AU  - Baker JV
FAU - Blumberg, Benjamin M
AU  - Blumberg BM
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030415
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antigens, Viral)
SB  - IM
CIN - J Infect Dis. 2003 May 1;187(9):1360-4. PMID: 12717616
MH  - Adult
MH  - Age of Onset
MH  - Antigens, Viral/*analysis
MH  - Brain/pathology/virology
MH  - Central Nervous System Infections/complications/pathology/virology
MH  - Female
MH  - *Genome, Viral
MH  - Herpesvirus 6, Human/genetics/*isolation & purification/pathogenicity
MH  - Humans
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Multiple Sclerosis/*etiology/pathology/*virology
MH  - Roseolovirus Infections/*complications/*diagnosis/pathology/virology
EDAT- 2003/04/30 05:00
MHDA- 2003/06/05 05:00
CRDT- 2003/04/30 05:00
PHST- 2002/08/07 00:00 [received]
PHST- 2002/12/02 00:00 [accepted]
PHST- 2003/04/30 05:00 [pubmed]
PHST- 2003/06/05 05:00 [medline]
PHST- 2003/04/30 05:00 [entrez]
AID - JID20942 [pii]
AID - 10.1086/368172 [doi]
PST - ppublish
SO  - J Infect Dis. 2003 May 1;187(9):1365-76. doi: 10.1086/368172. Epub 2003 Apr 15.