PMID- 12717618
OWN - NLM
STAT- MEDLINE
DCOM- 20030604
LR  - 20071115
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 187
IP  - 9
DP  - 2003 May 1
TI  - High frequency of human herpesvirus 6 DNA in multiple sclerosis plaques isolated 
      by laser microdissection.
PG  - 1377-87
AB  - The frequency of human herpesvirus 6 (HHV-6) DNA was assessed in autopsy material 
      from multiple sclerosis (MS) plaques and normal-appearing white matter (NAWM) 
      from brains of persons with MS, healthy brains, and brains of persons with other 
      neurologic diseases. Specific areas from formalin-fixed, paraffin-embedded brain 
      tissue samples were isolated by laser microscope. DNA was extracted from laser 
      microdissected brain material, and HHV-6 genomic sequences were amplified by 
      nested polymerase chain reaction. We analyzed 44 NAWM samples and 64 MS plaques 
      from 13 patients with MS, 46 samples from 13 patients with non-MS neurologic 
      disorders, and 41 samples from 12 healthy control brains. Of the 44 NAWM samples, 
      7 (15.9%) were positive for HHV-6 DNA sequences, versus 37 (57.8%) of 64 MS 
      plaques (P<.0005). HHV-6 DNA was detected in 10 (21.7%) of 46 samples from 
      patients with non-MS neurologic disorders and in 11 (26.8%) of 41 samples from 
      patients without known neurologic disease. Although the frequency of HHV-6 DNA 
      did not differ significantly by sample type, HHV-6 DNA was significantly more 
      common in MS plaques, suggesting that HHV-6 may play a role in MS pathogenesis.
FAU - Cermelli, Claudio
AU  - Cermelli C
AD  - Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, 
      National Institutes of Health, Bethesda, Maryland 20892, USA.
FAU - Berti, Rossana
AU  - Berti R
FAU - Soldan, Samantha S
AU  - Soldan SS
FAU - Mayne, Michael
AU  - Mayne M
FAU - D'ambrosia, James M
AU  - D'ambrosia JM
FAU - Ludwin, Samuel K
AU  - Ludwin SK
FAU - Jacobson, Steven
AU  - Jacobson S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030409
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (DNA, Viral)
SB  - IM
CIN - J Infect Dis. 2003 May 1;187(9):1360-4. PMID: 12717616
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain/pathology/virology
MH  - DNA, Viral/*analysis
MH  - Dissection/*methods
MH  - Female
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Laser Therapy/*methods
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/etiology/*pathology/*virology
MH  - Polymerase Chain Reaction
MH  - Roseolovirus Infections/complications/pathology/virology
MH  - Sensitivity and Specificity
EDAT- 2003/04/30 05:00
MHDA- 2003/06/05 05:00
CRDT- 2003/04/30 05:00
PHST- 2002/08/07 00:00 [received]
PHST- 2002/12/02 00:00 [accepted]
PHST- 2003/04/30 05:00 [pubmed]
PHST- 2003/06/05 05:00 [medline]
PHST- 2003/04/30 05:00 [entrez]
AID - JID20943 [pii]
AID - 10.1086/368166 [doi]
PST - ppublish
SO  - J Infect Dis. 2003 May 1;187(9):1377-87. doi: 10.1086/368166. Epub 2003 Apr 9.