PMID- 12719537
OWN - NLM
STAT- MEDLINE
DCOM- 20030701
LR  - 20220318
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 100
IP  - 10
DP  - 2003 May 13
TI  - Characterization of a family of endogenous neuropeptide ligands for the G 
      protein-coupled receptors GPR7 and GPR8.
PG  - 6251-6
AB  - GPR7 and GPR8 are orphan G protein-coupled receptors that are highly similar to 
      each other. These receptors are expressed predominantly in brain, suggesting 
      roles in central nervous system function. We have purified an endogenous peptide 
      ligand for GPR7 from bovine hypothalamus extracts. This peptide, termed 
      neuropeptide B (NPB), has a C-6-brominated tryptophan residue at the N terminus. 
      It binds and activates human GPR7 or GPR8 with median effective concentrations 
      (EC(50)) of 0.23 nM and 15.8 nM, respectively. In situ hybridization shows 
      distinct localizations of the prepro-NPB mRNA in mouse brain, i.e., in 
      paraventricular hypothalamic nucleus, hippocampus, and several nuclei in midbrain 
      and brainstem. Intracerebroventricular (i.c.v.) injection of NPB in mice induces 
      hyperphagia during the first 2 h, followed by hypophagia. Intracerebroventricular 
      injection of NPB produces analgesia to s.c. formalin injection in rats. Through 
      EST database searches, we identified a putative paralogous peptide. This peptide, 
      termed neuropeptide W (NPW), also has an N-terminal tryptophan residue. Synthetic 
      human NPW binds and activates human GPR7 or GPR8 with EC(50) values of 0.56 nM 
      and 0.51 nM, respectively. The expression of NPW mRNA in mouse brain is confined 
      to specific nuclei in midbrain and brainstem. These findings suggest diverse 
      physiological functions of NPB and NPW in the central nervous system, acting as 
      endogenous ligands on GPR7 andor GPR8.
FAU - Tanaka, Hirokazu
AU  - Tanaka H
AD  - Howard Hughes Medical Institute and Department of Molecular Genetics, University 
      of Texas Southwestern Medical Center, Dallas 75390-9050, USA.
FAU - Yoshida, Tetsuo
AU  - Yoshida T
FAU - Miyamoto, Norimasa
AU  - Miyamoto N
FAU - Motoike, Toshiyuki
AU  - Motoike T
FAU - Kurosu, Hiroshi
AU  - Kurosu H
FAU - Shibata, Kenji
AU  - Shibata K
FAU - Yamanaka, Akihiro
AU  - Yamanaka A
FAU - Williams, S Clay
AU  - Williams SC
FAU - Richardson, James A
AU  - Richardson JA
FAU - Tsujino, Natsuko
AU  - Tsujino N
FAU - Garry, Mary G
AU  - Garry MG
FAU - Lerner, Michael R
AU  - Lerner MR
FAU - King, David S
AU  - King DS
FAU - O'Dowd, Brian F
AU  - O'Dowd BF
FAU - Sakurai, Takeshi
AU  - Sakurai T
FAU - Yanagisawa, Masashi
AU  - Yanagisawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030428
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Ligands)
RN  - 0 (NPBWR1 protein, human)
RN  - 0 (NPBWR2 protein, human)
RN  - 0 (NPW protein, human)
RN  - 0 (Neuropeptides)
RN  - 0 (Npbwr1 protein, mouse)
RN  - 0 (Npbwr1 protein, rat)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Neuropeptide)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cattle
MH  - Conserved Sequence
MH  - GTP-Binding Proteins/*metabolism
MH  - Humans
MH  - Ligands
MH  - Melanophores/physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neuropeptides/chemistry/genetics/*metabolism
MH  - Rats
MH  - Receptors, G-Protein-Coupled
MH  - Receptors, Neuropeptide/genetics/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - Xenopus laevis
MH  - Zebrafish
PMC - PMC156358
EDAT- 2003/04/30 05:00
MHDA- 2003/07/02 05:00
PMCR- 2003/11/13
CRDT- 2003/04/30 05:00
PHST- 2003/04/30 05:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2003/04/30 05:00 [entrez]
PHST- 2003/11/13 00:00 [pmc-release]
AID - 0837789100 [pii]
AID - 7789 [pii]
AID - 10.1073/pnas.0837789100 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2003 May 13;100(10):6251-6. doi: 
      10.1073/pnas.0837789100. Epub 2003 Apr 28.