PMID- 12720349
OWN - NLM
STAT- MEDLINE
DCOM- 20030908
LR  - 20061115
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 75
IP  - 9
DP  - 2003 May 1
TI  - Specific aptamer-protein interaction studied by atomic force microscopy.
PG  - 2112-6
AB  - Aptamers are a new class of synthetic DNA/RNA oligonucleotides generated from in 
      vitro selection to selectively bind with various molecules. Due to their 
      molecular recognition capability for proteins, aptamers are becoming promising 
      reagents in protein detection and new drug development. In this study, the 
      specific interaction between the protein immunoglobulin E (IgE) and its 37-nt 
      aptamer has been measured directly by atomic force microscopy. The 
      single-molecule unbinding force between IgE and the aptamer is determined using 
      the Poisson statistical method. The individual unbinding force between IgE and 
      its monoclonal antibody has also been obtained and compared to that between IgE 
      and the aptamer. The results reveal the high affinity of the aptamer to protein, 
      which could match or even surpass that of the antibody to its antigen.
FAU - Jiang, Yaxin
AU  - Jiang Y
AD  - Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
FAU - Zhu, Chuanfeng
AU  - Zhu C
FAU - Ling, Liansheng
AU  - Ling L
FAU - Wan, Lijun
AU  - Wan L
FAU - Fang, Xiaohong
AU  - Fang X
FAU - Bai, Chunli
AU  - Bai C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Oligonucleotides)
RN  - 0 (Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Humans
MH  - Immunoglobulin E/analysis
MH  - Microscopy, Atomic Force
MH  - Oligonucleotides/*chemistry
MH  - Poisson Distribution
MH  - Proteins/*chemistry
EDAT- 2003/05/02 05:00
MHDA- 2003/09/10 05:00
CRDT- 2003/05/02 05:00
PHST- 2003/05/02 05:00 [pubmed]
PHST- 2003/09/10 05:00 [medline]
PHST- 2003/05/02 05:00 [entrez]
AID - 10.1021/ac026182s [doi]
PST - ppublish
SO  - Anal Chem. 2003 May 1;75(9):2112-6. doi: 10.1021/ac026182s.