PMID- 12721416
OWN - NLM
STAT- MEDLINE
DCOM- 20030801
LR  - 20190910
IS  - 0273-2289 (Print)
IS  - 0273-2289 (Linking)
VI  - 105 -108
DP  - 2003 Spring
TI  - Partition behavior and partial purification of hexokinase in aqueous two-phase 
      polyethylene glycol/citrate systems.
PG  - 787-97
AB  - This study dealt with the partition behavior and partial purification of 
      hexokinase (HK) from baker's yeast by liquid-liquid extraction using aqueous 
      two-phase polyethylene glycol (PEG)/citrate systems. First, we investigated the 
      effect of agitation type (vortex and 8 rpm rotation) on the stability of the 
      system, and then the effects of sodium citrate concentration, PEG concentration, 
      and molar mass of PEG on the partition coefficient of this enzyme by using a 25 
      factorial experimental design. The results of this factorial experiment showed 
      the possibility of a partial purification of HK by using two extraction steps, 
      since the enzyme preferentially migrated to the top phase and the total proteins 
      (mainly contaminants) remained in the bottom phase. The purification factor 
      (PurTOP) of the enzyme in the top phase was 1.87, and the partition coefficient 
      of the total proteins (KProt) was 0.47.
FAU - Oliveira, George G G
AU  - Oliveira GG
AD  - Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmacy, 
      University of Sao Paulo, Av. Prof. Lineu Prestes, 580, B.16. 05508-900, Sao 
      Paulo, SP, Brazil.
FAU - Silva, Daniel P
AU  - Silva DP
FAU - Roberto, Ines Conceicao
AU  - Roberto IC
FAU - Vitolo, Michele
AU  - Vitolo M
FAU - Pessoa, Adalberto Jr
AU  - Pessoa A Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Citrates)
RN  - 0 (Indicators and Reagents)
RN  - 059QF0KO0R (Water)
RN  - 1Q73Q2JULR (Sodium Citrate)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Citrates
MH  - Enzyme Stability
MH  - Hexokinase/*isolation & purification/*metabolism
MH  - Indicators and Reagents
MH  - Kinetics
MH  - Molecular Weight
MH  - Polyethylene Glycols
MH  - Saccharomyces cerevisiae/*enzymology
MH  - Sodium Citrate
MH  - Water
EDAT- 2003/05/02 05:00
MHDA- 2003/08/02 05:00
CRDT- 2003/05/02 05:00
PHST- 2003/05/02 05:00 [pubmed]
PHST- 2003/08/02 05:00 [medline]
PHST- 2003/05/02 05:00 [entrez]
AID - ABAB-108-1-3-787 [pii]
AID - 10.1385/abab:108:1-3:787 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2003 Spring;105 -108:787-97. doi: 
      10.1385/abab:108:1-3:787.