PMID- 12721791
OWN - NLM
STAT- MEDLINE
DCOM- 20040224
LR  - 20200228
IS  - 1434-5161 (Print)
IS  - 1434-5161 (Linking)
VI  - 48
IP  - 6
DP  - 2003
TI  - GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma 
      cell lines.
PG  - 331-335
LID - 10.1007/s10038-003-0026-2 [doi]
AB  - Comparative genomic hybridization (CGH) analyses have detected gains of copy 
      number on 13q, especially at 13q31-q32, in cell lines and primary cases of 
      various types of lymphoma. Since amplification of chromosomal DNA is one of the 
      mechanisms that can activate tumor-associated genes, and because 13q 
      amplification had been reported in various other types of tumors as well, we 
      attempted to define by fluorescence in situ hybridization (FISH) a common region 
      at 13q31-q32 in which to explore genes that might be targets for the 
      amplification events. Although the commonly amplified region we defined was 
      relatively large (approximately 4 Mb), only one true gene, GPC5, was found there. 
      GPC5 was over-expressed in lymphoma cell lines that had shown amplification, in 
      comparison with those that had not. Our findings suggest that GPC5 is a likely 
      target for amplification, and that over-expression of this gene may contribute to 
      development and/or progression of lymphomas and other tumors.
FAU - Yu, Wei
AU  - Yu W
AD  - Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical 
      and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
FAU - Inoue, Jun
AU  - Inoue J
AD  - Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical 
      and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
AD  - Theranostics Research Center (TRC), Otsuka Pharmaceutical Co., 463-10 Kagasuno 
      Kawauchi-cho, Tokushima 771-0192, Japan.
FAU - Imoto, Issei
AU  - Imoto I
AD  - Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical 
      and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
AD  - Core Research for Evolutional Science and Technology (CREST), Japan Science and 
      Technology Corporation (JST), 4-1-8 Honmachi, Kawaguchi 332-0012, Japan.
FAU - Matsuo, Yoshinobu
AU  - Matsuo Y
AD  - Fujisaki Cell Center, Hayashibara Biochemical Laboratories, 675-1 Fujisaki, 
      Okayama 702-8006, Japan.
FAU - Karpas, Abraham
AU  - Karpas A
AD  - Department of Hematology, University of Cambridge Clinical School, MRC Centre, 
      Hills Road, Cambridge, CB2 2QH, United Kingdom.
FAU - Inazawa, Johji
AU  - Inazawa J
AD  - Department of Molecular Cytogenetics, Medical Research Institute, Tokyo Medical 
      and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. 
      johinaz.cgen@mri.tmd.ac.jp.
AD  - Core Research for Evolutional Science and Technology (CREST), Japan Science and 
      Technology Corporation (JST), 4-1-8 Honmachi, Kawaguchi 332-0012, Japan. 
      johinaz.cgen@mri.tmd.ac.jp.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030429
PL  - England
TA  - J Hum Genet
JT  - Journal of human genetics
JID - 9808008
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (GPC5 protein, human)
RN  - 0 (Glypicans)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - *DNA, Neoplasm
MH  - Extracellular Matrix Proteins
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - Glypicans
MH  - Heparan Sulfate Proteoglycans/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma/*genetics/metabolism/pathology
MH  - RNA, Messenger/analysis
EDAT- 2003/05/02 05:00
MHDA- 2004/02/26 05:00
CRDT- 2003/05/02 05:00
PHST- 2003/02/14 00:00 [received]
PHST- 2003/03/13 00:00 [accepted]
PHST- 2003/05/02 05:00 [pubmed]
PHST- 2004/02/26 05:00 [medline]
PHST- 2003/05/02 05:00 [entrez]
AID - 10.1007/s10038-003-0026-2 [pii]
AID - 10.1007/s10038-003-0026-2 [doi]
PST - ppublish
SO  - J Hum Genet. 2003;48(6):331-335. doi: 10.1007/s10038-003-0026-2. Epub 2003 Apr 
      29.