PMID- 12721844
OWN - NLM
STAT- MEDLINE
DCOM- 20031003
LR  - 20181113
IS  - 0032-0935 (Print)
IS  - 0032-0935 (Linking)
VI  - 217
IP  - 1
DP  - 2003 May
TI  - Effects of carbohydrate starvation on gene expression in citrus root.
PG  - 11-20
AB  - The roots of alternate-bearing citrus (Murcott, a Citrus reticulata hybrid) trees 
      undergo extreme fluctuations of carbohydrate abundance and starvation. Using this 
      system, we investigated the effect of root carbohydrate (total soluble sugar, 
      sucrose and starch) depletion on carbohydrate-related gene expression. A series 
      of genes, including those coding for starch phosphorylase ( STPH-L and STPH-H), 
      ADP-glucose pyrophosphorylase, small subunit ( Agps), R1, plastidic ADP/ATP 
      transporter ( AATP), phosphoglucomutase ( PGM-P and PGM-C), sucrose synthase ( 
      CitSuS1 and CitSuSA), sucrose transporter ( SUT1 and SUT2), hexokinase ( HK) and 
      alpha-amylase ( alpha-AMY), have been isolated and their expression analyzed. The 
      genes were found to respond differentially to carbohydrate depletion. STPH-L, 
      STPH-H, Agps, R1, AATP, PGM-P, PGM-C, CitSuS1 and HK were down-regulated while 
      SUT1 and alpha-AMY were up-regulated during carbohydrate depletion. Two other 
      genes, CitSuSA and SUT2, did not respond to carbohydrate depletion. Fruit 
      removal, which interrupted the carbohydrate depletion induced by heavy fruiting, 
      reversed these gene expression patterns. Trunk girdling and whole-plant darkening 
      treatments, which brought about root carbohydrate depletion, induced the same 
      changes in gene expression obtained in the alternate-bearing system. The possible 
      roles of the up- and down-regulated genes in the metabolism of 
      carbohydrate-depleted citrus roots are discussed. Although the specific signals 
      involved have not been determined, the results support the feast/famine 
      hypothesis of carbohydrate regulation proposed by Koch [K.E. Koch (1996) Annu Rev 
      Plant Physiol Plant Mol Biol 47:509-540].
FAU - Li, Chun Yao
AU  - Li CY
AD  - The Institute of Plant Sciences and Genetics in Agriculture, Faculty of 
      Agricultural, Food, and Environmental Quality Sciences, The Hebrew University of 
      Jerusalem, 76100, Rehovot, Israel.
FAU - Weiss, David
AU  - Weiss D
FAU - Goldschmidt, Eliezer E
AU  - Goldschmidt EE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030128
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (Enzymes)
RN  - 57-50-1 (Sucrose)
RN  - 9005-25-8 (Starch)
SB  - IM
MH  - *Carbohydrate Metabolism
MH  - Citrus/genetics/*metabolism
MH  - Darkness
MH  - Enzymes/genetics
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Plant
MH  - Microscopy, Electron, Scanning
MH  - Plant Roots/genetics/*metabolism/ultrastructure
MH  - Plant Stems/genetics/metabolism
MH  - Starch/metabolism
MH  - Sucrose/metabolism
EDAT- 2003/05/02 05:00
MHDA- 2003/10/04 05:00
CRDT- 2003/05/02 05:00
PHST- 2002/06/24 00:00 [received]
PHST- 2002/11/26 00:00 [accepted]
PHST- 2003/05/02 05:00 [pubmed]
PHST- 2003/10/04 05:00 [medline]
PHST- 2003/05/02 05:00 [entrez]
AID - 10.1007/s00425-002-0963-6 [doi]
PST - ppublish
SO  - Planta. 2003 May;217(1):11-20. doi: 10.1007/s00425-002-0963-6. Epub 2003 Jan 28.